Wang S Q, Jiang G L, Wei G M, Huo F M, Dong L L, Zhao L P, Huang H R, Wang G R
National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing 101149, China.
Zhonghua Jie He He Hu Xi Za Zhi. 2018 Jul 12;41(7):539-543. doi: 10.3760/cma.j.issn.1001-0939.2018.07.007.
To investigate the antimicrobial susceptibility and genotyping of . A total of 150 . isolates were collected. The susceptibility against 15 antimicrobial agents widely used for treatment of non-tuberculosis mycobacteria (NTM) infections, was tested by broth microdilution assay. Variable number of tandem repeats (VNTR) assay was also performed using the 16-loci genotyping method. The drug susceptibility test revealed that clarithromycin (97.3%, 146/150), moxifloxacin (94.0%, 141/150) and amikacin (90.0%, 135/150) had the best antimicrobial activities against the . isolates. Secondly, 75.3%(113/150), 64.0%(96/150), 52.7%(79/150) and 8.7%(13/150) of the strains were susceptible to rifampicin, linezolid, capreomycin, and ethambutol, respectively. The MIC(50) and MIC(90) values of the 3 injectable anti-tuberculosis drugs were as follows: amikacin 4 mg/L and 16 mg/L, streptomycin 4 mg/L and 16 mg/L, capreomycin 8 mg/L and 16 mg/L. The MIC(50) and MIC(90) values of the 5 different fluoroquinolones were 0.5 mg/L and 2 mg/L for moxifloxacin , 1 mg/L and 8 mg/L for ciprofloxacin, 1 mg/L and 8ug/ml for levofloxacin, 2 mg/L and 16 mg/L for antoflolxacin, 2 mg/L and 16 mg/L for ofloxacin. The Hunter-Gaston Discriminatory Index (HGDI) value for the 16-loci VNTR typing of . isolates was 0.994. VNTR differentiated the 150 isolates into 21 clusters and acquired a total of 121 unique patterns. Drug resistance profile was not independently associated with cluster strains. Clarithromycin, moxifloxacin and amikacin had the best antimicrobial activities against . isolates. The 16-loci VNTR typing revealed a highly discriminatory power and drug resistance profile was not independently associated with cluster strains.
为研究……的抗菌药敏性和基因分型。共收集了150株……分离株。采用肉汤微量稀释法检测了15种广泛用于治疗非结核分枝杆菌（NTM）感染的抗菌药物的敏感性。还使用16位点基因分型方法进行了可变数目串联重复序列（VNTR）分析。药敏试验显示，克拉霉素（97.3%，146/150）、莫西沙星（94.0%，141/150）和阿米卡星（90.0%，135/150）对……分离株具有最佳抗菌活性。其次，分别有75.3%（113/150）、64.0%（96/150）、52.7%（79/150）和8.7%（13/150）的菌株对利福平、利奈唑胺、卷曲霉素和乙胺丁醇敏感。3种注射用抗结核药物的MIC（50）和MIC（90）值如下：阿米卡星4mg/L和16mg/L，链霉素4mg/L和16mg/L，卷曲霉素8mg/L和16mg/L。5种不同氟喹诺酮类药物的MIC（50）和MIC（90）值分别为：莫西沙星0.5mg/L和2mg/L，环丙沙星1mg/L和8mg/L，左氧氟沙星1mg/L和8μg/ml，安妥沙星2mg/L和16mg/L，氧氟沙星2mg/L和16mg/L。……分离株16位点VNTR分型的Hunter-Gaston鉴别指数（HGDI）值为0.994。VNTR将150株分离株分为21个簇，共获得121种独特模式。耐药谱与簇菌株无独立相关性。克拉霉素、莫西沙星和阿米卡星对……分离株具有最佳抗菌活性。16位点VNTR分型显示出高度的鉴别力，耐药谱与簇菌株无独立相关性。
