Zhang W Y, Xuan B, Guo Y X, Zhang J
Department of Prosthodontics, Stomatological Hospital, Tianjin Medical University, Tianjin 300070, China.
Department of Prosthodontics, Stomatological Hospital, Tianjin Medical University, Tianjin 300070, China (Present address: Department of Stomatology, Aerospace Central Hospital, Beijing 100039, China).
Zhonghua Kou Qiang Yi Xue Za Zhi. 2018 Jul 9;53(7):466-469. doi: 10.3760/cma.j.issn.1002-0098.2018.07.007.
To further study the effects of distal-less homeobox gene 5 (Dlx-5) and Msh homeobox 1 (Msx-1) in the pathogenic mechanism of bisphosphonate related osteonecrosis of the jaw (BRONJ) . Twenty-four SD rats were divided into two groups, the experimental group was injected intraperitoneally with zoledronic acid for 12 weeks (0.2 mg/kg, three times a week), and the control group was injected with saline solution for 12 weeks. The first mandibular molars were extracted after 12 weeks. All of the animals were sacrificed eight weeks after teeth extraction. The BRONJ was diagnosed by gross observation, X-ray examination and histopathlolgical examination. Through real-time PCR, the expression level of Dlx-5 and Msx-1 were detected in the mandible of BRONJ samples and normal samples. X-ray examination and histopathlolgical analysis showed the presence of BRONJ. The results of real-time PCR showed that the expression levels of Dlx-5 were increased (0.001) and the expression level of Msx-1 was decreased (0.001) in the experimental group compared with the control group. Dlx-5 and Msx-1 genes play roles in the pathogenic mechanism of BRONJ.
为进一步研究远端缺失同源盒基因5（Dlx-5）和Msh同源盒1（Msx-1）在双膦酸盐相关颌骨坏死（BRONJ）发病机制中的作用。将24只SD大鼠分为两组，实验组腹腔注射唑来膦酸12周（0.2mg/kg，每周3次），对照组注射生理盐水12周。12周后拔除第一下颌磨牙。拔牙8周后处死所有动物。通过大体观察、X线检查和组织病理学检查诊断BRONJ。通过实时PCR检测BRONJ样本和正常样本下颌骨中Dlx-5和Msx-1的表达水平。X线检查和组织病理学分析显示存在BRONJ。实时PCR结果显示，与对照组相比，实验组中Dlx-5的表达水平升高（P<0.001），Msx-1的表达水平降低（P<0.001）。Dlx-5和Msx-1基因在BRONJ的发病机制中起作用。
