Gibson T R, Glembotski C C
Peptides. 1985 Jul-Aug;6(4):615-20. doi: 10.1016/0196-9781(85)90162-7.
ACTH(1-8) and ACTH(9-13)NH2 were used as potential enzyme inhibitors to begin examining the relationship between the acetylation of ACTH- and beta-endorphin-related peptides. ACTH(1-8) was a potent inhibitor of the acetylation of both ACTH- and beta-endorphin-related peptides, whereas ACTH(9-13)NH2 was an effective inhibitor only of the acetylation of ACTH-related substrates. This inhibition pattern indicated that there may be an unusual interaction between some ACTH- and beta-endorphin-related peptides as substrates for the acetyltransferase. Utilizing HPLC to separate ACTH- and beta-endorphin-related peptides present in the same reaction mixture, ACTH(1-14) and beta-endorphin(1-27) at Km and saturating concentrations were used as substrates to examine the ability of one peptide substrate to affect the acetylation of the other. It was observed that the acetylation of ACTH(1-14), even at Km concentration, was relatively unaffected by the presence of beta-endorphin(1-27). However, the acetylation of beta-endorphin(1-27) was significantly reduced by the presence of ACTH(1-14). This preferential acetylation of ACTH-related peptides over the acetylation of beta-endorphin-related peptides might have physiological importance under some conditions.
促肾上腺皮质激素（1-8）和促肾上腺皮质激素（9-13）氨基被用作潜在的酶抑制剂，以开始研究促肾上腺皮质激素和β-内啡肽相关肽的乙酰化之间的关系。促肾上腺皮质激素（1-8）是促肾上腺皮质激素和β-内啡肽相关肽乙酰化的有效抑制剂，而促肾上腺皮质激素（9-13）氨基仅是促肾上腺皮质激素相关底物乙酰化的有效抑制剂。这种抑制模式表明，作为乙酰转移酶底物的一些促肾上腺皮质激素和β-内啡肽相关肽之间可能存在异常相互作用。利用高效液相色谱法分离存在于同一反应混合物中的促肾上腺皮质激素和β-内啡肽相关肽，将处于米氏常数（Km）和饱和浓度的促肾上腺皮质激素（1-14）和β-内啡肽（1-27）用作底物，以研究一种肽底物影响另一种肽乙酰化的能力。观察到，即使在米氏常数浓度下，β-内啡肽（1-27）的存在对促肾上腺皮质激素（1-14）的乙酰化相对没有影响。然而，促肾上腺皮质激素（1-14）的存在显著降低了β-内啡肽（1-27）的乙酰化。在某些情况下，促肾上腺皮质激素相关肽的这种优先乙酰化相对于β-内啡肽相关肽的乙酰化可能具有生理重要性。
