Davis G S, Siopes T D
Poult Sci. 1985 Nov;64(11):2189-94. doi: 10.3382/ps.0642189.
In each of two trials, plasma corticosterone (B) was measured in Large White turkey tom poults after the following treatments were applied: 1) .9% saline injected; 2) cold water immersion, and 3) adrenocorticotropic hormone (ACTH) injected (10 IU/kg body weight). Poults were treated at 3- to 4-day intervals from the day of hatching to 21 days of age. Plasma samples were obtained at 1, 2, 3, 4, and 6 hr posttreatment. In both trials, there was a depression in B levels within the first 3 hr following ACTH or cold water immersion treatment. Significant increases in plasma B levels of the cold water treatment occurred at 4 hr posttreatment in Trial 1 in 7-day-old poults and in Trial 2 in 21-day-old poults. A significant adrenal cortical response to ACTH injection was observed in 3- and 7-day-old poults at 6 hrs posttreatment in Trial 2. Plasma B concentrations were also measured in three groups of nontreated Large White tom poults on the day of hatching at a commercial hatchery. Plasma samples were obtained from poults in incubators at 1000 hr, immediately following commercial processing procedures at 1030 hr, and at poult placement at 1330 hr. Plasma B levels of poults sampled in the incubator and after processing were similar. However, B levels of poults sampled at placement were increased significantly above the other two groups.
在两项试验中,对大白火鸡公雏进行以下处理后测量其血浆皮质酮(B)水平:1)注射0.9%生理盐水;2)冷水浸泡;3)注射促肾上腺皮质激素(ACTH,10 IU/kg体重)。从孵化日至21日龄,每隔3至4天对雏鸡进行一次处理。在处理后1、2、3、4和6小时采集血浆样本。在两项试验中,ACTH或冷水浸泡处理后的前3小时内,B水平均有所下降。在试验1中,7日龄雏鸡在处理后4小时,以及试验2中,21日龄雏鸡在处理后4小时,冷水处理组的血浆B水平显著升高。在试验2中,3日龄和7日龄雏鸡在注射ACTH后6小时观察到显著的肾上腺皮质反应。在商业孵化场,还对三组未处理的大白火鸡公雏在孵化日当天测量了血浆B浓度。在1000时从孵化器中的雏鸡采集血浆样本,在1030时按照商业处理程序后立即采集,以及在1330时雏鸡放置时采集。在孵化器中采样的雏鸡和处理后的血浆B水平相似。然而,放置时采样的雏鸡的B水平显著高于其他两组。