Nowak Paweł Mateusz, Olesek Katarzyna, Woźniakiewicz Michał, Kościelniak Paweł
Jagiellonian University in Kraków, Faculty of Chemistry, Department of Analytical Chemistry, Kraków, Poland.
Electrophoresis. 2018 Oct;39(19):2406-2409. doi: 10.1002/elps.201800142. Epub 2018 Jul 25.
Methcathinone (ephedrone), 4-methylmethcathinone (mephedrone), and 3-methylmethcathinone (metaphedrone) are toxicologically-important cathinone derivatives used commonly as designer drugs. In this work we show the first method allowing to separate simultaneously all these molecules in a chiral medium, ensuring good resolution between all enantiomers. Eight cyclodextrins have been tested as potential chiral selectors, the best results were obtained with 2-hydroxyethyl-β-cyclodextrin, unreported so far for efficient separation of cathinones. After optimization, the method was calibrated and validated with and without the use of internal standard. The addition of standard improved an overall repeatability and precision, the use of electrophoretic mobility ratio was especially favorable (RSD < 1%). It was demonstrated that the method may be easily extended by introducing the additional cathinone-related drugs to the sample, maintaining satisfactory separation efficiency.
去甲伪麻黄碱（麻黄酮）、4-甲基去甲伪麻黄碱（甲基麻黄碱）和3-甲基去甲伪麻黄碱（甲基苯丙胺）是毒理学上重要的卡西酮衍生物，通常用作设计药物。在这项工作中，我们展示了第一种能够在手性介质中同时分离所有这些分子的方法，确保了所有对映体之间的良好分离度。八种环糊精作为潜在的手性选择剂进行了测试，使用2-羟乙基-β-环糊精获得了最佳结果，到目前为止，该环糊精尚未被报道可有效分离卡西酮。经过优化后，该方法在使用和不使用内标的情况下进行了校准和验证。标准品的加入提高了整体重复性和精密度，使用电泳迁移率比尤其有利（相对标准偏差<1%）。结果表明，通过向样品中引入其他与卡西酮相关的药物，该方法可以很容易地扩展，同时保持令人满意的分离效率。
