Jonsson G G, Eriksson G, Pero R W
Radiat Res. 1985 May;102(2):241-53.
Effects of hyperthermia (42.5 degrees C) and gamma radiation (30 Gy) on ADP-ribosyl transferase, NAD+, and ATP pools in human mononuclear leukocytes have been investigated. It was found that the gamma-ray activation level of the enzyme was not influenced by this hyperthermia for 45 min. Following deprivation of ATP synthesis by 2,4-dinitrophenol, an uncoupler of the oxidative phosphorylation, and omitting glucose from the culture medium, the NAD+ pool was reduced to about 60% of control value. The potentiation of ATP production by exogenously supplied adenosine was reduced after a combined treatment of the cells with hyperthermia and gamma radiation. Mitochondrial and endoplasmic changes within the mononuclear leukocytes were also observed. Based on these findings a model for the hyperthermia effect is proposed.
研究了热疗（42.5摄氏度）和γ辐射（30戈瑞）对人单核白细胞中ADP-核糖基转移酶、NAD⁺和ATP池的影响。发现该酶的γ射线激活水平不受45分钟热疗的影响。在用氧化磷酸化解偶联剂2,4-二硝基苯酚剥夺ATP合成并从培养基中省略葡萄糖后，NAD⁺池减少到对照值的约60%。在用热疗和γ辐射联合处理细胞后，外源性供应腺苷增强ATP产生的作用降低。还观察到单核白细胞内的线粒体和内质网变化。基于这些发现，提出了一个热疗效应模型。
