Kawasaki Akane, Yasuda Makoto, Mawatari Ken-Ichi, Fukuuchi Tomoko, Yamaoka Noriko, Kaneko Kiyoko, Iijima Ryosuke, Yui Satoru, Satoh Motonobu, Nakagomi Kazuya
Faculty of Pharma-Science, Teikyo University.
Anal Sci. 2018;34(7):841-844. doi: 10.2116/analsci.18N001.
N-Acetylneuraminic acid (NANA) has been reported to react with hydrogen peroxide in vitro to produce 4-(acetylamino)-2,4-dideoxy-D-glycero-D-galacto-octonic acid (ADOA). We labeled NANA and ADOA with 4-(N,N-dimethylaminosulfonyl)-7-piperazino-2,1,3-benzoxadiazole (DBD-PZ) for simultaneous detection. The derivatized NANA and ADOA were separated using hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection. The calibration curves of DBD-PZ-derivatized NANA and ADOA showed good linearity in the range of 221 fmol to 1.5 nmol, and 44 fmol to 1.5 nmol, respectively. This analytical method has high specificity and is useful for the detection of NANA and ADOA in saliva and serum.
据报道，N-乙酰神经氨酸（NANA）在体外与过氧化氢反应生成4-（乙酰氨基）-2,4-二脱氧-D-甘油-D-半乳糖辛酸（ADOA）。我们用4-（N，N-二甲基氨基磺酰基）-7-哌嗪基-2,1,3-苯并恶二唑（DBD-PZ）标记NANA和ADOA以进行同时检测。衍生化的NANA和ADOA采用亲水相互作用液相色谱（HILIC）结合荧光检测进行分离。DBD-PZ衍生化的NANA和ADOA的校准曲线在221 fmol至1.5 nmol和44 fmol至1.5 nmol范围内分别显示出良好的线性。该分析方法具有高特异性，可用于检测唾液和血清中的NANA和ADOA。
