[Identification and Characterzation of the Porcine Epidemic Diarrhea Virus (PEDV) Isolates].

To explore the porcine epidemic diarrhea virus(PEDV)epidemiology in domestic pigs, virus isolation and identification were carried out in pig herds in Shanghai and Jiangsu province. Based on sequences of PEDV strains in GenBank, a pair of primers targeting the M gene were designed for PEDV detection. Two clinical pig diarrhea samples were amplified and sequencing results confirmed that the fragments amplified were M gene. PEDV-positive samples were inoculated in Vero cells with different concentrations of trypsin. Our results showed that these two isolates could proliferate effectively in Vero cells with a specific concentration of trypsin. Twenty-second passages viruses were added to the feeding of newborn piglets, and animal regression test showed obvious clinical symptoms of diarrhea, confirming the the PEDV isolates, named as JSLS/PEDV/1/2014 and JS/PEDV/2/2014,were successfully obtained. Phylogenetic analysis of the M genes showed that JSLS/PEDV/1/2014 and JS/PEDV/2/2014 displayed the greatest similarity with the Chinese strains HLJ-2012 and BJ-2012-1,respectively.The S genes of the two isolates were classified in group I. There were 51 bases deletion in the ORF3 genes of these two isolates which were located in the same big branch with the DR13 strain rather than the CV777 strain.