Laboratory of General Microbiology, Department of Biology of Bacteria, Institute of Microbiology, Biotechnology and Immunology, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland.
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991 Moscow, Russia.
Int J Biol Macromol. 2018 Oct 15;118(Pt A):1131-1135. doi: 10.1016/j.ijbiomac.2018.06.174. Epub 2018 Jul 2.
The current serological classification scheme of the medically important bacteria from the genus Proteus consists of 80 O serogroups, the last four of which (O77-O80) were created from clinical strains from Łódź, Poland. There are more serologically unique strains isolated from patient that do not fit into the existing scheme, such as Proteus mirabilis strain Sm 99 isolated from urine of a 74-year-old woman in Łódź. Serological investigation involving ELISA and Western blotting failed to classify the Proteus mirabilis strain Sm 99 into any of the 80 Proteus O serogroups. Sugar analysis along with two-dimensional NMR spectroscopy showed that the O-polysaccharide is composed of branched pentasaccharide repeating units containing one residue each of d-Glc, d-GlcNAc, d-GalNAc, d-glucuronic acid, and 4-[(R)-3-hydroxybutanoylamino]-4,6-dideoxy-d-glucose. The chemical and serological data show that the O antigen of P. mirabilis Sm 99 is unique among the known Proteus O antigens. Based on this finding, it is proposed to extend the current serological classification scheme of Proteus by adding a new serogroup, O81, which at present consists of P. mirabilis strain Sm 99 only.
目前，医学上重要的变形杆菌属细菌的血清分类方案包括 80 个 O 血清群，其中最后四个（O77-O80）是由波兰罗兹的临床菌株创建的。还有更多从患者中分离出来的血清学独特的菌株，无法归入现有的分类方案，例如从罗兹 74 岁女性尿液中分离出来的奇异变形杆菌 Sm 99 菌株。涉及 ELISA 和 Western blotting 的血清学调查未能将奇异变形杆菌 Sm 99 菌株归入 80 个变形杆菌 O 血清群中的任何一个。糖分析以及二维 NMR 光谱表明，O-多糖由支链五糖重复单元组成，每个单元含有一个 d-Glc、d-GlcNAc、d-GalNAc、d-葡萄糖醛酸和 4-[(R)-3-羟基丁酰氨基]-4,6-二脱氧-d-葡萄糖。化学和血清学数据表明，P. mirabilis Sm 99 的 O 抗原在已知的变形杆菌 O 抗原中是独特的。基于这一发现，建议通过添加一个新的血清群 O81 来扩展变形杆菌的现行血清分类方案，目前该血清群仅由奇异变形杆菌 Sm 99 菌株组成。
