Luengo J M
Anal Biochem. 1985 Sep;149(2):466-70. doi: 10.1016/0003-2697(85)90599-8.
An easy, rapid, and available method for separating 6-aminopenicillanic acid (6-APA), benzylpenicillin (penicillin G), and other related molecules from aqueous solutions or complex industrial broths is described. A high concentration of ammonium sulphate induces partially or totally the precipitation of the penicillin present in the solutions, while 6-APA, phenylacetic, and phenoxyacetic acid always remain in the supernatant. The filtration through No. 4 Pyrex glass-fiber filter or Whatman 3MM paper permits the separation of the compounds present in the supernatant from the other ones precipitated. The precipitated product was identified, in all cases, as ammonium penicillin. This method is described here for the first time.
本文描述了一种从水溶液或复杂工业发酵液中分离6-氨基青霉烷酸（6-APA）、苄青霉素（青霉素G）及其他相关分子的简便、快速且可行的方法。高浓度硫酸铵可部分或完全诱导溶液中青霉素沉淀，而6-APA、苯乙酸和苯氧乙酸则始终留在上清液中。通过4号派热克斯玻璃纤维滤纸或沃特曼3MM滤纸过滤，可将上清液中的化合物与其他沉淀的化合物分离。在所有情况下，沉淀产物均被鉴定为青霉素铵。此方法为首创。
