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来自……的……的分子克隆及特性分析

Molecular cloning and characteristics analysis of from .

作者信息

Hao Ruijuan, Zheng Zhe, Du Xiaodong, Wang Qingheng, Li Junhui, Deng Yuewen, Chen Weiyao

机构信息

Fisheries College, Guangdong Ocean University, Zhanjiang, 524088, China.

Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang, 524088, China.

出版信息

Biotechnol Rep (Amst). 2018 Jun 5;19:e00262. doi: 10.1016/j.btre.2018.e00262. eCollection 2018 Sep.

Abstract

is cultured for pearl production. Growth improvement has received considerable research interest. Transforming growth factor β type Ⅰ receptor (TβR-I), which is involved in signals transmission of transforming growth factor beta (TGF-β), participates in cell proliferation and growth. In this study, we characterized a gene which encoded TβR-I from (). cDNA contains an open reading frame of 1569 bp and encodes a polypeptide of 522 amino acids (aa). Pmtgfbr1 possesses a typical TβR-I structure (extracellular receptor ligand domain, transmembrane domain, and cytoplasmic tyrosine kinase catalytic domain). is expressed in all the studied tissues and exhibited the highest expression level in the adductor muscle. Moreover, exhibited the lower expression level in the larger group (L) than that in the smaller group (S) and is negatively correlated with growth traits ( < 0.01). Our results indicated that is a candidate functional gene associated with growth traits.

摘要

用于珍珠生产养殖。生长改良受到了相当多的研究关注。参与转化生长因子β(TGF-β)信号传导的转化生长因子βⅠ型受体(TβR-I)参与细胞增殖和生长。在本研究中,我们鉴定了一个从()中编码TβR-I的基因。cDNA包含一个1569 bp的开放阅读框,编码一个522个氨基酸(aa)的多肽。Pmtgfbr1具有典型的TβR-I结构(细胞外受体配体结构域、跨膜结构域和细胞质酪氨酸激酶催化结构域)。在所有研究组织中均有表达,在内收肌中表达水平最高。此外,在较大组(L)中的表达水平低于较小组(S),且与生长性状呈负相关(<0.01)。我们的结果表明,是与生长性状相关的候选功能基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4c1/6041369/9f484d260e39/gr1.jpg

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