Faghani Masoumeh, Keshavars Parvaneh, Sharafshah Alireza, Pourgholamali Babak, Moharami Farshad, Nemati Shadman
Anatomical Sciences Department, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
Eur Arch Otorhinolaryngol. 2018 Sep;275(9):2253-2257. doi: 10.1007/s00405-018-5059-4. Epub 2018 Jul 12.
Nasal Polyposis (NP) is a complex multi-factorial disease; associated with several environmental, genetic and inflammatory factors. TNF-alpha is one of the major pro-inflammatory cytokines involved in NP pathogenesis. Some of the polymorphisms of this gene affect its expression. Therefore, the aim of this study was to evaluate the polymorphism of TNF-alpha G/A308 gene and its association with nasal polyposis in north part of Iran. In this case-control study, 60 patients with NP and 75 healthy individuals referred to Guilan University hospital were evaluated. After DNA extraction, RFLP-PCR was used to determine polymorphism. Chi-square test was used to compare the frequency distribution of genotype and alleles of TNF-alpha gene with NP. The frequency of genotype G/G, A/A and G/A in the NP group was 46.7, 10.0 and 43.3%, and in the control group was 65.3, 5.3 and 29.3%, respectively. There was a statistically significant difference between genotype G/G in two groups (P = 0.006, OR 2.58, CI 1.27-5.23). In addition, the frequency of allele A in patients and controls was 31.7 and 20%, respectively; and this difference was statistically significant (p = 0.02). The findings of this study demonstrated that polymorphism in TNF-alpha gene might be a risk factor for NP in north part of Iran and the minor frequency of G308A allele in the current study is slightly more than other major populations. However, more investigations with high number of population are necessary in future.
鼻息肉病（NP）是一种复杂的多因素疾病，与多种环境、遗传和炎症因素相关。肿瘤坏死因子-α（TNF-α）是参与NP发病机制的主要促炎细胞因子之一。该基因的一些多态性会影响其表达。因此，本研究的目的是评估TNF-α基因G/A308位点的多态性及其与伊朗北部鼻息肉病的相关性。在这项病例对照研究中，对转诊至吉兰大学医院的60例NP患者和75名健康个体进行了评估。提取DNA后，采用限制性片段长度多态性聚合酶链反应（RFLP-PCR）来确定多态性。采用卡方检验比较NP患者与对照组中TNF-α基因的基因型和等位基因频率分布。NP组中基因型G/G、A/A和G/A的频率分别为46.7%、10.0%和43.3%，对照组中分别为65.3%、5.3%和29.3%。两组间基因型G/G存在统计学显著差异（P = 0.006，OR 2.58，CI 1.27 - 5.23）。此外，患者和对照组中等位基因A的频率分别为31.7%和20%，这一差异具有统计学显著性（p = 0.02）。本研究结果表明，TNF-α基因多态性可能是伊朗北部NP的一个危险因素，且本研究中G308A等位基因的次要频率略高于其他主要人群。然而，未来有必要进行更多大规模人群的调查。
