Department of Chemistry, Sardar Patel University, Vallabh Vidyanagar, Gujarat, 388120, India.
Appl Biochem Biotechnol. 2019 Feb;187(2):556-569. doi: 10.1007/s12010-018-2835-y. Epub 2018 Jul 13.
Half sandwich complexes of the type [(η5-CMe)M(L)Cl]Cl.2HO were synthesized using [{(η-CMe)M(μ-Cl)Cl}], where M = Rh(III)/Ir(III) and L = pyrimidine-based ligands. The complexes were characterized by spectral analysis. DNA interaction studies by absorption titration and hydrodynamic measurement and suggest intercalative mode of binding of complexes with CT-DNA. The molecular docking study also supports intercalation of the complexes between the stacks of nucleotide base pairs. The gel electrophoresis assay demonstrated the ability of the complexes to interact and cleave plasmid DNA. Minimum inhibitory concentrations (MIC) of the complexes were investigated by the microdilution broth method. The cytotoxic properties of the metal complexes were evaluated using brine shrimp lethality bioassay.
采用 [{(η-CMe)M(μ-Cl)Cl}] 合成了 [(η5-CMe)M(L)Cl]Cl.2HO 型半三明治配合物，其中 M = Rh(III)/Ir(III)，L = 嘧啶基配体。通过光谱分析对配合物进行了表征。通过吸收滴定和流体力学测量的 DNA 相互作用研究表明，配合物与 CT-DNA 以嵌入模式结合。分子对接研究也支持配合物在核苷酸碱基对之间的堆积中嵌入。凝胶电泳实验证明了配合物与质粒 DNA 相互作用和切割的能力。采用微量稀释肉汤法测定配合物的最小抑菌浓度 (MIC)。通过盐水虾致死生物测定评价金属配合物的细胞毒性。
