Sumimoto H, Takeshige K, Minakami S
Biochem Biophys Res Commun. 1985 Nov 15;132(3):864-70. doi: 10.1016/0006-291x(85)91887-x.
Human polymorphonuclear leukocytes, but not mononuclear leukocytes, platelets, or erythrocytes, oxidized 20-hydroxy-leukotriene B4 (20-OH-LTB4) to 20-carboxy-LTB4 (20-COOH-LTB4). 20-OH-LTB4 was quantitatively converted to 20-COOH-LTB4 by the sonicate of polymorphonuclear leukocytes in the presence of NAD+, with an optimal pH of about 7.9. NADP+ could not replace NAD+. The conversion was not inhibited by 2 mM pyrazole, a potent inhibitor of alcohol dehydrogenase. When LTB4 was incubated with the sonicate in the presence of NADPH and NAD+, 20-OH-LTB4 and 20-COOH-LTB4 appeared concomitantly with the disappearance of LTB4.
人多形核白细胞而非单核白细胞、血小板或红细胞可将20-羟基白三烯B4（20-OH-LTB4）氧化为20-羧基白三烯B4（20-COOH-LTB4）。在NAD⁺存在的情况下，多形核白细胞的超声提取物可将20-OH-LTB4定量转化为20-COOH-LTB4，最适pH约为7.9。NADP⁺不能替代NAD⁺。2 mM吡唑（一种有效的乙醇脱氢酶抑制剂）不会抑制这种转化。当白三烯B4在NADPH和NAD⁺存在的情况下与超声提取物一起孵育时，20-OH-LTB4和20-COOH-LTB4会随着白三烯B4的消失而同时出现。
