Ding Xiaoqing, Chen Haiyan
Department of Hematology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, 100078, China.
J BUON. 2018 May-Jun;23(3):747-751.
There is accumulating evidence indicating that plant-derived monoterpenes have impressive health-promoting effects, among them anticancer, antimicrobial and many other activities. Carvone is an important monoterpene with pharmacological potential. In the current study we investigated the anticancer effects of carvone in myeloma KMS-5 cells.
The cell viability was estimated by MTT assay. Apoptosis was detected by DAPI, acridine orange/ethidium bromide (AO/EB) and annexin V/propidium iodide (PI) staining. Cell cycle analysis was done by flow cytometry. Cell invasion was determined by martigel invasion assay and protein expression was checked by western blotting.
The results of the present study indicated carvone exerts significant anticancer effects on the myeloma cancer cells in a dose-dependent manner. The IC50 of carvone was found to be 20 μM against the myeloma KMS-5 cells. The antiproliferative effects were due to induction of apoptosis and G2/M cell cycle arrest. Moreover, carvone could inhibit the cell invasion and the expression of p-P38 protein at IC50.
In conclusion, the results indicate that carvone could prove to be an important molecule for the development of natural product-based anticancer therapy, especially against myeloma.
越来越多的证据表明，植物来源的单萜具有显著的促进健康作用，其中包括抗癌、抗菌和许多其他活性。香芹酮是一种具有药理潜力的重要单萜。在本研究中，我们研究了香芹酮对骨髓瘤KMS-5细胞的抗癌作用。
通过MTT法评估细胞活力。通过DAPI、吖啶橙/溴化乙锭（AO/EB）和膜联蛋白V/碘化丙啶（PI）染色检测细胞凋亡。通过流式细胞术进行细胞周期分析。通过基质胶侵袭试验测定细胞侵袭，并通过蛋白质印迹法检测蛋白质表达。
本研究结果表明，香芹酮对骨髓瘤癌细胞具有显著的抗癌作用，且呈剂量依赖性。发现香芹酮对骨髓瘤KMS-5细胞的IC50为20μM。其抗增殖作用归因于诱导细胞凋亡和G2/M期细胞周期阻滞。此外，香芹酮在IC50时可抑制细胞侵袭和p-P38蛋白的表达。
总之，结果表明香芹酮可能是开发基于天然产物的抗癌疗法，尤其是抗骨髓瘤疗法的重要分子。
