Zhu Yun, Rao Qiu, Zhang Xinhua, Zhou Xiaojun
Department of Pathology, Nanjing Jinling Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, 210002, China.
J BUON. 2018 May-Jun;23(3):795-799.
Galangin is an important flavonoid that has been reported to be of immense pharmacological importance. It has been shown to have a number of bioactivities which range from anticancer to antimicrobial. In this study the effects of Galangin were studied on the proliferation of the A498 kidney cancer cells.
The antiproliferative effects were tested by MTT assay. Apoptosis was checked by subjecting the A498 cell to DAPI and annexin V/PI double staining. The effect on the cell migration and invasion was assessed by Boyden chamber assays. The expression of the apoptosis-related proteins was examined by western blot analysis.
Galangin inhibited the proliferation of the kidney cancer A498 cells. The IC50 of Galangin against the A498 cancer cells was 15 μM. The anticancer effects of Galangin were due to induction of apoptosis in the A498 cancer cells as indicated by DAPI and annexin V/PI staining. Furthermore, Galangin could increase the expression of Bax, Cyt-c and decrease the expression of Bcl-2. Galangin could also inhibit the migration and invasion of the kidney cancer cells and also suppress the expression of some of the important proteins of the PI3K/AKT/mTOR signalling pathway.
In conclusion, Galangin could prove a useful new molecule in the treatment of kidney carcinoma.
高良姜素是一种重要的黄酮类化合物，据报道具有巨大的药理学重要性。已表明它具有多种生物活性，范围从抗癌到抗菌。在本研究中，研究了高良姜素对A498肾癌细胞增殖的影响。
通过MTT法测试抗增殖作用。通过对A498细胞进行DAPI和膜联蛋白V/PI双重染色来检查细胞凋亡。通过Boyden小室试验评估对细胞迁移和侵袭的影响。通过蛋白质印迹分析检测凋亡相关蛋白的表达。
高良姜素抑制肾癌细胞A498的增殖。高良姜素对A498癌细胞的IC50为15μM。如DAPI和膜联蛋白V/PI染色所示，高良姜素的抗癌作用是由于诱导A498癌细胞凋亡。此外，高良姜素可增加Bax、细胞色素c的表达并降低Bcl-2的表达。高良姜素还可抑制肾癌细胞的迁移和侵袭，并抑制PI3K/AKT/mTOR信号通路中一些重要蛋白的表达。
总之，高良姜素可能是治疗肾癌的一种有用的新分子。
