Konstantopoulou Kallirroi, Tsiambas Evangelos, Baliou Evangelia, Lazaris Andreas C, Kavantzas Nikolaos, Karameris Andreas, Fotiades Panagiotis P, Asproudis Ioannis, Ragos Vasileios, Vilaras George, Patsouris Efstratios, Konstantopoulou Kallirroi, Tsiambas Evangelos, Baliou Evangelia, Lazaris Andreas C, Kavantzas Nikolaos, Karameris Andreas, Fotiades Panagiotis P, Asproudis Ioannis, Ragos Vasileios, Vilaras George, Patsouris Efstratios
Department of Ophthalmology, General Hospital, Kalamata, Greece.
J BUON. 2018 May-Jun;23(3):826-831.
Pterygium is a distinct clinicopathological entity characterized by degenerated and neoplastic-like features. Concerning its rise on normal conjunctiva epithelia, the role of specific gene deregulations including apoptotic/anti-apoptotic factors and significant suppressor genes in signaling transduction pathways is under investigation. In the current study, we co-analyzed p53, survivin and PTEN proteins in pterygia and normal conjunctiva.
Using a liquid-based cytology assay, 50 cell specimens were obtained by a smooth scraping on conjunctiva epithelia and fixed accordingly. Among them, 38 were pterygia and the remaining (n=12) normal epithelia (control group). Immunocytochemistry assays were implemented on the corresponding slides by applying ani-p53, survivin, and PTEN antibodies. Digital image analysis was performed for evaluating objectively the corresponding immunostaining intensity levels.
The majority of the examined pterygia cases overexpressed the markers p53:22/38-57.9%, survivin:30/38-78.9%, and PTEN:25/38-65.7%. Interestingly, overall p53/PTEN co-expression was found to be statistically significant (p=0.022).
Survivin overexpression leads to an increased anti-apoptotic activity playing a central molecular role in the pathogenesis and progression of pterygia. Furthermore, although p53 expression is observed in these lesions, its impact seems to be low compared to survivin's influence on them. Additionally, the role of PTEN in the process is potentially significant providing a suppressor balance to the p53/ survivin complex.
翼状胬肉是一种具有退化和肿瘤样特征的独特临床病理实体。关于其在正常结膜上皮上的发生，包括凋亡/抗凋亡因子和信号转导途径中重要抑制基因在内的特定基因失调的作用正在研究中。在本研究中，我们对翼状胬肉和正常结膜中的p53、生存素和PTEN蛋白进行了联合分析。
采用液基细胞学检测法，通过在结膜上皮上平滑刮取获得50个细胞标本并进行相应固定。其中，38个为翼状胬肉，其余12个为正常上皮（对照组）。通过应用抗p53、生存素和PTEN抗体在相应载玻片上进行免疫细胞化学检测。进行数字图像分析以客观评估相应的免疫染色强度水平。
大多数受检翼状胬肉病例中标记物p53、生存素和PTEN呈过表达，p53：22/38 - 57.9%，生存素：30/38 - 78.9%，PTEN：25/38 - 65.7%。有趣的是，总体p53/PTEN共表达具有统计学意义（p = 0.022）。
生存素过表达导致抗凋亡活性增加，在翼状胬肉的发病机制和进展中起核心分子作用。此外，尽管在这些病变中观察到p53表达，但其影响与生存素对它们的影响相比似乎较低。此外，PTEN在该过程中的作用可能很重要，为p53/生存素复合物提供抑制平衡。
