Gao Fangfang, Lu Wenhui, Liu Huitao, Li Jinhua, Chen Lingxin
College of Chemistry and Chemical Engineering, Yantai University, Yantai, P. R. China.
CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Center for Coastal Environmental Engineering Technology of Shandong Province, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, P. R. China.
Electrophoresis. 2018 Oct;39(19):2431-2438. doi: 10.1002/elps.201800205. Epub 2018 Jul 26.
Dispersive liquid-liquid microextraction (DLLME) coupled with CE was developed for simultaneous determination of five types of chlorophenols (CPs), namely 2-chlorophenol (2-CP), 4-chlorophenol (4-CP), 2,4-dichlorophenol (2,4-DCP), 2,6-dichlorophenol (2,6-DCP), and 2,4,6-trichlorophenol (2,4,6-TCP) in water samples. Several parameters affecting DLLME and CE conditions were systematically investigated. Under the optimized DLLME-CE conditions, the five CPs were separated completely within 7.5 min and good enrichment factors were obtained of 40, 193, 102, 15, and 107 for 4-CP, 2,4,6-TCP, 2,4-DCP, 2-CP, and 2,6-DCP, respectively. Good linearity was attained in the range of 1-200 μg/L for 2,4,6-TCP, 2,4-DCP, 2-300 μg/L for 4-CP and 2-CP, and 1-300 μg/L for 2,6-DCP, with correlation coefficients (r) over 0.99. The LOD (S/N = 3) and the LOQ (S/N = 10) were 0.31-0.75 μg/L and 1.01-2.43 μg/L, respectively. Recoveries ranging from 60.85 to 112.36% were obtained with tap, lake, and river water spiked at three concentration levels and the RSDs (for n = 3) were 1.31-11.38%. With the characteristics of simplicity, cost-saving, and environmental friendliness, the developed DLLME-CE method proved to be potentially applicable for the rapid, sensitive, and simultaneous determination of trace CPs in complicated water samples.
建立了分散液液微萃取（DLLME）与毛细管电泳（CE）联用的方法，用于同时测定水样中的5种氯酚（CPs），即2 - 氯酚（2 - CP）、4 - 氯酚（4 - CP）、2,4 - 二氯酚（2,4 - DCP）、2,6 - 二氯酚（2,6 - DCP）和2,4,6 - 三氯酚（2,4,6 - TCP）。系统研究了影响DLLME和CE条件的几个参数。在优化的DLLME - CE条件下，5种CPs在7.5分钟内完全分离，4 - CP、2,4,6 - TCP、2,4 - DCP、2 - CP和2,6 - DCP的富集因子分别为40、193、102、15和107。2,4,6 - TCP、2,4 - DCP、2 - CP在1 - 200μg/L范围内，4 - CP和2 - CP在2 - 300μg/L范围内，2,6 - DCP在1 - 300μg/L范围内具有良好的线性，相关系数（r）均大于0.99。检测限（S/N = 3）和定量限（S/N = 10）分别为0.31 - 0.75μg/L和1.01 - 2.43μg/L。对自来水、湖水和河水进行三个浓度水平的加标回收试验，回收率在60.85%至112.36%之间，相对标准偏差（n = 3）为1.31% - 11.38%。所建立的DLLME - CE方法具有简单、经济和环境友好的特点，被证明可潜在地应用于复杂水样中痕量CPs的快速、灵敏和同时测定。
