Hu Zongyue, Xu Zhouheng, Lu Yiyu
Bing Du Xue Bao. 2016 Sep;32(5):659-65.
Loop mediated isothermal amplification(LAMP)technology is a newly developed isothermal amplification technology for in vitro detection of nucleic acids. Although the LAMP assay is rapid, specific, sensitive, simple and has been widely applied for rapid detection of nucleic acids, it continues to improve and develop. In this paper, we summarize approaches to addressing amplification product contamination, primer design to avoid false positives, and the development of related techniques based on LAMP technology. This paper could serve as a reference for the application of the assay at the grassroots level.
环介导等温扩增（LAMP）技术是一种新开发的用于体外核酸检测的等温扩增技术。尽管LAMP检测快速、特异、灵敏、简便，已广泛应用于核酸的快速检测，但它仍在不断改进和发展。本文总结了应对扩增产物污染的方法、避免假阳性的引物设计以及基于LAMP技术的相关技术的发展。本文可为该检测方法在基层的应用提供参考。
