Wang Haiyan, Tao Zexin, Li Yan, Zhang Wenqiang, Liu Xiaolin, Xu Aiqiang
Bing Du Xue Bao. 2016 Nov;32(6):683-8.
To investigate the molecular epidemiological characterization of coxsackieivirus B1(CV-B1),we performed VP1 sequencing on all isolates from acute flaccid paralysis (AFP) surveillance, environmental surveillance and viral meningitis specimens from 1994 to 2015.A total of 53CV-B1 strains were isolated, among which 41 strains,4strains,and 8strains were obtained from AFP surveillance, environmental surveillance and meningitis specimens, respectively. Phylogenetic analysis based on VP1 entire coding region revealed that Shandong CV-B1 strains were segregated into a major cluster alongside with other domestic strains, with no foreign strains existing in this cluster. Foreign strains composed two other exclusive branches.Shandong strains had VP1 nucleotide similarities of 84.4%to 100.0% with each other, and 77.9%to 85.0% with foreign strains. The results presented here demonstrate Chinese CV-B1 strains have great genetic divergence with foreign strains. EV associated surveillance should be reinforced to monitor possible importation of different EV transmission lineages.
为研究柯萨奇病毒B1（CV-B1）的分子流行病学特征，我们对1994年至2015年急性弛缓性麻痹（AFP）监测、环境监测及病毒性脑膜炎标本中的所有分离株进行了VP1基因测序。共分离出53株CV-B1毒株，其中分别从AFP监测、环境监测和脑膜炎标本中获得41株、4株和8株。基于VP1完整编码区的系统发育分析显示，山东CV-B1毒株与其他国内毒株一起被归入一个主要聚类，该聚类中不存在国外毒株。国外毒株构成另外两个独立分支。山东毒株彼此间VP1核苷酸相似性为84.4%至100.0%，与国外毒株的相似性为77.9%至85.0%。此处结果表明中国CV-B1毒株与国外毒株存在很大的遗传差异。应加强肠道病毒相关监测，以监测不同肠道病毒传播谱系可能的输入情况。
