Department of Anesthesiology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, 833, Taiwan.
Department of Cardiology, Wei-Gong Memorial Hospital, Miaoli, 351, Taiwan.
Sci Rep. 2018 Jun 11;8(1):8821. doi: 10.1038/s41598-018-27254-y.
Coxsackievirus (CV)-B5 is a common human enterovirus reported worldwide; swine vesicular disease virus (SVDV) is a porcine variant of CV-B5. To clarify the transmission dynamics and molecular basis of host switching between CV-B5 and SVDV, we analysed and compared the VP1 and partial 3D gene regions of these two viruses. Spatiotemporal dynamics of viral transmission were estimated using a Bayesian statistical inference framework. The detected selection events were used to analyse the key molecules associated with host switching. Analyses of VP1 sequences revealed six CV-B5 genotypes (A1-A4 and B1-B2) and three SVDV genotypes (I-III). Analyses of partial 3D revealed five clusters (A-E). The genotypes evolved sequentially over different periods, albeit with some overlap. The major hub of CV-B5 transmission was in China whereas the major hubs of SVDV transmission were in Italy. Network analysis based on deduced amino acid sequences showed a diverse extension of the VP1 structural protein, whereas most sequences were clustered into two haplotypes in the partial 3D region. Residue 178 of VP1 showed four epistatic interactions with residues known to play essential roles in viral host tropism, cell entry, and viral decoating.
柯萨奇病毒（CV）-B5 是一种在世界范围内广泛报道的常见人类肠道病毒；猪水疱病病毒（SVDV）是 CV-B5 的猪变异株。为了阐明 CV-B5 和 SVDV 之间宿主转换的传播动态和分子基础，我们分析和比较了这两种病毒的 VP1 和部分 3D 基因区域。使用贝叶斯统计推断框架估计了病毒传播的时空动态。检测到的选择事件用于分析与宿主转换相关的关键分子。VP1 序列分析揭示了六种 CV-B5 基因型（A1-A4 和 B1-B2）和三种 SVDV 基因型（I-III）。部分 3D 分析揭示了五个聚类（A-E）。CV-B5 传播的主要中心在中国，而 SVDV 传播的主要中心在意大利。基于推导的氨基酸序列的网络分析显示，VP1 结构蛋白的广泛扩展，而大部分序列在部分 3D 区域聚类为两个单倍型。VP1 的 178 位残基与已知在病毒宿主嗜性、细胞进入和病毒脱壳中起重要作用的残基有四个上位相互作用。
