Chen Jianhua, Zhu Zhen, Li Yushan, Wu Haizhuo, Liu Haixia, Wang Xuxia, Yu Deshan
Bing Du Xue Bao. 2016 Nov;32(6):721-6.
We used molecular-biology methods to identify the pathogens that caused an outbreak of viral encephalitis in Guazhou (Gansu province, China)during June-August 2015.We also undertook molecular characterizations of these pathogens. A total of 132samples(14cerebrospinal fluid(CSF)samples;25throat swabs;66serum samples;27fecal samples)were collected from 74 patients during the outbreak of viral encephalitis. For CSF and serum samples, enzyme-linked immunosorbent assay immunoglobulin-M tests were undertaken to detect Japanese encephalitis viruses, enteroviruses, herpes simplex viruses, mumps viruses, and adenoviruses. Real-time polymerase chain reaction was done to detect enteroviruses(including coxsackievirus A16 and enterovirus 71) and the RNA of human adenoviruses. Then, viral isolation was carried out using HEp-2 and RD cells, and the entire VP1 region of positive viral isolates was amplified and sequenced. Finally, molecular characterizations of these pathogens were completed. Seventy two samples were identified as enteroviruses from 132 samples. Among them,71 were identified as echovirus(ECHO)30using enterovirus molecular typing. Japanese encephalitis viruses,herpes simplex viruses, mumps viruses, and adenoviruses were not detected.ECHO30 was isolated from 46 samples out of 29 patients.Similarities in nucleic acids among these ECHO30 isolates were 99.2%-100.0%.ECHO30 from Gansu province and other ECHO30 strains isolated in China since 2011 belonged to a same evolutionary branch.ECHO30 was the pathogen that caused the outbreak of viral encephalitis in Guazhou in 2015.ECHO30 from and Gansu province and ECHO30 isolated in China since 2011 belonged to the same evolutionary branch.
我们采用分子生物学方法鉴定了2015年6月至8月期间在中国甘肃省瓜州引发病毒性脑炎疫情的病原体。我们还对这些病原体进行了分子特征分析。在病毒性脑炎疫情期间，从74名患者身上共采集了132份样本（14份脑脊液样本、25份咽拭子、66份血清样本、27份粪便样本）。对于脑脊液和血清样本，进行了酶联免疫吸附测定免疫球蛋白-M试验，以检测日本脑炎病毒、肠道病毒、单纯疱疹病毒、腮腺炎病毒和腺病毒。采用实时聚合酶链反应检测肠道病毒（包括柯萨奇病毒A16和肠道病毒71）以及人腺病毒的RNA。然后，使用HEp-2和RD细胞进行病毒分离，并对阳性病毒分离株的整个VP1区域进行扩增和测序。最后，完成了这些病原体的分子特征分析。在132份样本中，有72份被鉴定为肠道病毒。其中，71份通过肠道病毒分子分型被鉴定为埃可病毒（ECHO）30。未检测到日本脑炎病毒、单纯疱疹病毒、腮腺炎病毒和腺病毒。从29名患者中的46份样本中分离出了ECHO30。这些ECHO30分离株之间的核酸相似度为99.2%-100.0%。甘肃省的ECHO30与自2011年以来在中国分离出的其他ECHO30毒株属于同一进化分支。ECHO30是2015年瓜州病毒性脑炎疫情的病原体。甘肃省的ECHO30与自2011年以来在中国分离出的ECHO30属于同一进化分支。
