Cao Xuefeng, Peng Guangneng, Huang Xiangming, Wang Wenbo, Tian Yinan, Xu Xiaoyang, Lei Shuangshuang, Yang Yanyi, Gong Chao, Yang Kuixing, Yuan Bo, Ren Zhihua, Zhong Zhijun
Bing Du Xue Bao. 2016 Nov;32(6):733-9.
We investigated infection by canine parvovirus and genetic variation of the VP2 gene. We collected feces samples of 50 diarrheal dogs in Sichuan Province, China. Analyses polymerase chain reaction (PCRs), agarose gel electrophoresis, and amplification of the complete sequence of canine parvovirus were done. We observed 19PCR-positive samples. Sequencing analyses of 15PCR-positive samples based on amplification of the complete VP2 gene showed all to be CPV-2a,and to be polymerized with Sichuan isolates. These results suggest that the common epidemic strain in Sichuan Province is CPV-2a,and may originate from the same strain. Compared with reference strains, there were no significant variations in canine parvovirus in Sichuan Province, China.
我们调查了犬细小病毒感染情况以及VP2基因的遗传变异。我们收集了中国四川省50只腹泻犬的粪便样本。进行了聚合酶链反应(PCR)分析、琼脂糖凝胶电泳以及犬细小病毒全序列扩增。我们观察到19个PCR阳性样本。基于完整VP2基因扩增对15个PCR阳性样本进行测序分析,结果显示均为CPV-2a,且与四川分离株聚合。这些结果表明,四川省的常见流行毒株为CPV-2a,且可能源自同一毒株。与参考毒株相比,中国四川省的犬细小病毒没有显著变异。