一种识别非回文序列的新型限制性内切酶Eco31I。

A new restriction endonuclease Eco31I recognizing a non-palindromic sequence.

作者信息

Butkus V, Bitinaité J, Kersulyté D, Janulaitis A

出版信息

Biochim Biophys Acta. 1985 Dec 18;826(4):208-12. doi: 10.1016/0167-4781(85)90008-9.

Abstract

A restriction endonuclease with a novel site-specificity has been isolated from the Escherichia coli strain RFL31. The nucleotide sequences around a single Eco31I cut on pBR322 DNA and two cuts of lambda DNA have been compared. A common 5'GAGACC 3'CTCTGG sequence occurs near each cleavage site. Precise mapping of the cleavages in both DNA strands places the cuts five nucleotides to the left of the upper sequence and one nucleotide to the left of the lower sequence. This enabled us to deduce the following recognition and cleavage specificity of Eco31I: 5' GGTCTCN decreases 3' CCAGAGN NNNN increases.

摘要

一种具有新型位点特异性的限制性内切酶已从大肠杆菌菌株RFL31中分离出来。已比较了pBR322 DNA上单个Eco31I切割位点周围以及λ DNA上两个切割位点周围的核苷酸序列。在每个切割位点附近都出现了一个共同的5'GAGACC 3'CTCTGG序列。对两条DNA链上切割位点的精确图谱分析表明，切割位点位于上部序列左侧五个核苷酸处，位于下部序列左侧一个核苷酸处。这使我们能够推断出Eco31I的以下识别和切割特异性：5' GGTCTCN减少 3' CCAGAGN NNNN增加。