Laboratorio de Nanocelulosa y Biomateriales, Departamento de Ingeniería Química, Biotecnología y Materiales, Facultad de Ciencias Físicas y Matemáticas , Universidad de Chile , Beauchef 851 , Santiago , Chile.
Department of Fibre and Polymer Technology, KTH , Royal Institute of Technology , Teknikringen 56-58 , 100 44 Stockholm , Sweden.
Biomacromolecules. 2018 Aug 13;19(8):3549-3559. doi: 10.1021/acs.biomac.8b00831. Epub 2018 Jul 26.
Protein-functionalized cellulose fibrils, having various amounts of covalently bonded proteins at their surface, were successfully extracted from the tunic of Pyura chilensis tunicates using successive alkaline extractions. Pure cellulose fibrils were also obtained by further bleaching and were used as reference material. Extraction yields of protein-functionalized cellulose fibrils were within the range of 62-76% by weight based on the dry initial tunic powder. Fourier-transform infrared and Raman spectroscopy confirmed the preservation of residual protein at the surface of cellulose fibrils, which was then quantified by X-ray photoelectron spectroscopy. The protein-functionalized cellulose fibrils were found to have relatively high crystallinity and their cellulose I crystalline structure was preserved upon applying alkaline treatments. The extracted cellulosic materials were found to be constituted of fibrils having a ribbon-like morphology with widths ranging from ∼30 nm up to ∼400 nm. These protein-functionalized cellulose fibrils were found to have outstanding thermal stability with one of them having onset and peak degradation temperatures of ∼350 and 374 °C, respectively. These values were found to be 24 and 41 °C higher than for bleached cellulose.
使用连续的碱性提取方法，成功地从智利海鞘的被囊中提取出具有不同数量的共价键合蛋白质的蛋白功能化纤维素纤维。通过进一步漂白也获得了纯纤维素纤维，并将其用作参考材料。基于干燥的初始被囊粉，蛋白功能化纤维素纤维的提取产率在 62-76%重量范围内。傅里叶变换红外和拉曼光谱证实了纤维素纤维表面上残留蛋白质的保留，然后通过 X 射线光电子能谱对其进行了定量。发现蛋白功能化纤维素纤维具有相对较高的结晶度，并且在施加碱性处理时其纤维素 I 结晶结构得以保留。所提取的纤维素材料由具有带状形态的纤维组成，宽度范围从约 30nm 到约 400nm。这些蛋白功能化纤维素纤维具有出色的热稳定性，其中一种的起始和峰值降解温度分别约为 350°C 和 374°C。这些值比漂白纤维素高 24°C 和 41°C。
