Jurczak Andrzej, Janowski Tomasz
Department of Animal Reproduction with Clinic, University of Warmia and Mazury, Olsztyn, Poland.
Department of Animal Reproduction with Clinic, University of Warmia and Mazury, Olsztyn, Poland.
Theriogenology. 2018 Oct 1;119:131-136. doi: 10.1016/j.theriogenology.2018.06.014. Epub 2018 Jun 22.
The aim of this study was to compare arterial blood flow determined by repeated Doppler sonographic examinations in the periovulatory period in GnRH-induced and spontaneous estrous cycles of bitches. The study was performed on 10 bitches with GnRH agonist implant-induced estrus (Suprelorin containing 4.7 mg deslorelin), and 10 bitches with spontaneous estrus (group B), which served as a control. Pulsed-wave Doppler ultrasonography was performed daily from the beginning of proestrus until early metestrus. The ovarian blood flow parameters in the left and right ovarian arteries, including systolic peak velocity (SPV), end diastolic velocity (EDV) and resistance index (RI), were measured. Obtained outcomes from the period of three days before ovulation (Days -3, -2, -1), ovulation (day 0) and three days after ovulation (Days +1, +2, +3) were evaluated statistically. There were no differences between blood flow variables in the bitches with spontaneous and induced cycles recorded on the same day. The values of SPV and EDV were higher (p < 0.05) on days -1, 0, +1, +2 and + 3 than the velocimetric variables collected on days -3 and -2. Then, a significant increase in blood flow was detected one day before ovulation and was continued during next 3 days (p < 0.05). In contrast, the mean RI values remained constant during the entire experiment or even showed a slight declining trend in both groups (p > 0.05). We concluded that arterial blood flow measured around ovulation does not allow for precise detection of exact ovulation time in bitches in induced and spontaneous cycles.
本研究的目的是比较在诱导发情周期和自然发情周期的排卵前期，通过重复多普勒超声检查测定的母犬动脉血流情况。本研究对10只使用GnRH激动剂植入物诱导发情的母犬（含有4.7mg去甲雄三烯醇酮的Suprelorin）和10只自然发情的母犬（B组）进行，后者作为对照。从发情前期开始至发情后期早期，每天进行脉冲波多普勒超声检查。测量左右卵巢动脉的卵巢血流参数，包括收缩期峰值流速（SPV）、舒张末期流速（EDV）和阻力指数（RI）。对排卵前三天（第-3、-2、-1天）、排卵日（第0天）和排卵后三天（第+1、+2、+3天）获得的结果进行统计学评估。在同一天记录的自然发情和诱导发情周期的母犬中，血流变量没有差异。在第-1、0、+1、+2和+3天，SPV和EDV的值高于在第-3和-2天收集的流速测量变量（p<0.05）。然后，在排卵前一天检测到血流显著增加，并在接下来的3天持续（p<0.05）。相比之下，在整个实验过程中，两组的平均RI值保持恒定，甚至呈轻微下降趋势（p>0.05）。我们得出结论，在诱导发情周期和自然发情周期的母犬中，排卵前后测量的动脉血流无法精确检测到确切的排卵时间。
