White M C, Adams E F, Loizou M, Mashiter K, Fahlbusch R
Clin Endocrinol (Oxf). 1985 Sep;23(3):295-302. doi: 10.1111/j.1365-2265.1985.tb00227.x.
We have investigated the effects of ovine corticotrophin releasing factor (oCRF) and its interaction with hydrocortisone (HC), and arginine vasopressin (AVP) on ACTH release from human corticotrophinoma cells in culture. Tumour tissue was obtained from six patients (three with active Cushing's disease and three with Nelson's syndrome). Cultures were maintained for periods of up to six months. Ovine CRF (21 nmol) significantly (P less than 0.01) stimulated ACTH release from all tumours. Dose response (21 pmol-21 nmol) effects were observed for the three tumours investigated over 2 and 4 h. Cortisol (20 mumol) significantly (P less than 0.01) inhibited basal ACTH release from one tumour (Nelson's syndrome) by 75% over 4 h, and completely prevented the stimulatory effects of oCRF. AVP directly stimulated ACTH release from two tumours (Nelson's syndrome), and also potentiated the stimulatory action of oCRF during 30 min incubations. These data show corticotrophinoma cells from subjects with Cushing's disease and Nelson's syndrome can be directly stimulated by hypothalamic oCRF and may be potentiated by AVP. Cortisol and oCRF have been shown in one tumour to have antagonistic actions at the pituitary level.
我们研究了羊促肾上腺皮质激素释放因子（oCRF）及其与氢化可的松（HC）和精氨酸加压素（AVP）的相互作用对培养的人促肾上腺皮质激素瘤细胞释放促肾上腺皮质激素（ACTH）的影响。肿瘤组织取自6例患者（3例患有活动性库欣病，3例患有尼尔森综合征）。培养物维持长达6个月。羊CRF（21 nmol）显著（P＜0.01）刺激所有肿瘤释放ACTH。在所研究的3个肿瘤中，在2小时和4小时观察到剂量反应（21 pmol - 21 nmol）效应。皮质醇（20 μmol）在4小时内显著（P＜0.01）抑制1个肿瘤（尼尔森综合征）的基础ACTH释放达75%，并完全阻断了oCRF的刺激作用。AVP直接刺激2个肿瘤（尼尔森综合征）释放ACTH，并且在30分钟的孵育期间增强了oCRF的刺激作用。这些数据表明，来自库欣病和尼尔森综合征患者的促肾上腺皮质激素瘤细胞可被下丘脑oCRF直接刺激，并且可能被AVP增强。在1个肿瘤中已表明皮质醇和oCRF在垂体水平具有拮抗作用。
