Ishii Akihiro, Han David, Bansal Rashmi
Department of Neuroscience, University of Connecticut Medical School, Farmington, CT, USA.
Methods Mol Biol. 2018;1791:67-77. doi: 10.1007/978-1-4939-7862-5_6.
The identification and functional characterization of the repertoire of myelin proteins provides a valuable foundation for understanding molecular mechanisms of myelination and the pathogenesis of human myelin disease. Here we provide a procedure for the purification of myelin from rodent or human brains and a large-scale analysis of the myelin proteome, using the shotgun approach of one-dimensional PAGE and liquid chromatography (LC)/tandem mass spectrometry (MS).
髓磷脂蛋白库的鉴定及其功能特性分析为理解髓鞘形成的分子机制以及人类髓鞘疾病的发病机理提供了重要基础。在此，我们提供了一种从啮齿动物或人类大脑中纯化髓磷脂的方法，并采用一维聚丙烯酰胺凝胶电泳（PAGE）和液相色谱（LC）/串联质谱（MS）的鸟枪法对髓磷脂蛋白质组进行大规模分析。
