Blasiak Agata, Nag Sudip, Yang In Hong
Singapore Institute for Neurotechnology, National University of Singapore, Singapore, Singapore.
Department of Electronics and Electrical Communication Engineering, Indian Institute of Technology, Kharagpur, India.
Methods Mol Biol. 2018;1791:207-224. doi: 10.1007/978-1-4939-7862-5_16.
Activity-dependent myelination modulates neuron conduction velocity and as such it is essential for a correct wiring of a whole nervous system. Increasing myelination through inducing neuron activity has been proposed as a treatment strategy for demyelination diseases. Yet, the mechanisms and the effects of activity-dependent myelination remain elusive-new tools are needed. In this chapter, we describe a novel compartmentalized device integrated with an optogenetic stimulator for studying activity-dependent myelination in vitro. The platform can be modified to include multiple cell types, stimulation modes, and experimental readouts to answer a specific research question. This versatility combined with a precise control over spatial extent of the stimulation and the stimulation pattern make the proposed platform a valuable tool for molecular myelination studies.
活动依赖型髓鞘形成调节神经元传导速度，因此对于整个神经系统的正确布线至关重要。通过诱导神经元活动来增加髓鞘形成已被提出作为脱髓鞘疾病的一种治疗策略。然而，活动依赖型髓鞘形成的机制和影响仍然难以捉摸——需要新的工具。在本章中，我们描述了一种与光遗传学刺激器集成的新型分隔装置，用于体外研究活动依赖型髓鞘形成。该平台可以进行修改，以包括多种细胞类型、刺激模式和实验读数，以回答特定的研究问题。这种多功能性与对刺激的空间范围和刺激模式的精确控制相结合，使所提出的平台成为分子髓鞘形成研究的有价值工具。
