Wang Chenyi, Kotter Mark R
Department of Clinical Neurosciences, Anne McLaren Laboratory, University of Cambridge, Cambridge, UK.
Methods Mol Biol. 2018;1791:233-241. doi: 10.1007/978-1-4939-7862-5_18.
Multiple sclerosis (MS) is a chronic autoimmune, inflammatory disease in the central nervous system (CNS) characterized by loss of oligodendrocytes, myelin axons, and neurons. Remyelination is an endogenous repair mechanism, which recovers the loss of myelin and is able to preserve functional axons. The hope is that the development of new treatments aiming at promoting remyelination will halt and potentially partially reverse the progressive neurological decline in MS. The development of such drugs requires adequate models. In this chapter, we will discuss the surgical procedure of injection of lysolecithin into ventral thoraco-lumbar spinal cord white matter of mice, which is particularly suitable for investigating remyelination using transgenic animals.
多发性硬化症（MS）是一种慢性自身免疫性炎症性疾病，发生于中枢神经系统（CNS），其特征为少突胶质细胞、髓鞘轴突和神经元的丢失。髓鞘再生是一种内源性修复机制，可恢复髓鞘的丢失并能够保留功能性轴突。人们希望开发旨在促进髓鞘再生的新疗法将阻止并可能部分逆转MS患者逐渐进展的神经功能衰退。此类药物的研发需要合适的模型。在本章中，我们将讨论向小鼠胸腰段脊髓腹侧白质注射溶血卵磷脂的手术操作，该操作特别适用于使用转基因动物研究髓鞘再生。
