髓磷脂蛋白零肽诱导C57BL/6小鼠实验性自身免疫性神经炎。

Myelin Protein Zero Peptide Induced Experimental Autoimmune Neuritis in C57BL/6 Mice.

作者信息

Gonsalvez David G, Yoo SangWon, Craig Georgina A, Wood Rhiannon J, Fletcher Jessica L, Murray Simon S, Xiao Junhua

机构信息

Faculty of Medicine, Dentistry and Health Sciences, Department of Anatomy and Neuroscience, School of Biomedical Sciences, The University of Melbourne, Melbourne, VIC, Australia.

出版信息

Methods Mol Biol. 2018;1791:243-250. doi: 10.1007/978-1-4939-7862-5_19.

DOI:10.1007/978-1-4939-7862-5_19

PMID:30006715

Abstract

Mouse models of peripheral demyelinating neuropathy play an important role in enabling the study of disease pathogenesis. Further, induction in transgenic mice allows for the precise interrogation of disease mechanisms, as well as the analysis of the efficacy and mechanisms of potential new therapies. Here we describe a method to successfully induce experimental autoimmune neuritis (EAN) using myelin protein zero (P0) peptide in combination with Freund's complete adjuvant and pertussis toxin in the C57BL/6 mouse strain. We also outline a sensitive paradigm of accurately assessing the extent of functional deficits occurring in murine EAN.

摘要

外周脱髓鞘性神经病的小鼠模型在疾病发病机制的研究中发挥着重要作用。此外，在转基因小鼠中诱导发病能够精确探究疾病机制，还能分析潜在新疗法的疗效及作用机制。在此，我们描述一种在C57BL/6小鼠品系中使用髓鞘蛋白零（P0）肽联合弗氏完全佐剂和百日咳毒素成功诱导实验性自身免疫性神经炎（EAN）的方法。我们还概述了一种准确评估小鼠EAN中功能缺陷程度的灵敏方案。