Dun Xin-Peng, Parkinson David B
Plymouth University Peninsula Schools of Medicine and Dentistry, Plymouth, Devon, UK.
Methods Mol Biol. 2018;1791:251-262. doi: 10.1007/978-1-4939-7862-5_20.
Injury to the peripheral nervous system begins a well-characterized process within both neurons and Schwann cells to allow axonal regrowth, remyelination, and functional repair. Models of peripheral nerve injury have been widely used to study the behavior of Schwann cells, neurons, and other cell types such as macrophages as the events of Wallerian degeneration and regeneration take place. The most commonly used approaches in rodent models to model nerve injury in human patients are sciatic nerve transection and nerve crush, and both have well established time courses of demyelination, immune cell influx, axonal regrowth, and remyelination. We describe the techniques of sciatic nerve surgery for transection and crush injury, together with methods for the analysis of events within peripheral nerve repair in these two models.
外周神经系统损伤会在神经元和施万细胞内启动一个特征明确的过程，以促进轴突再生、髓鞘再生和功能修复。由于沃勒变性和再生过程的发生，外周神经损伤模型已被广泛用于研究施万细胞、神经元以及其他细胞类型（如巨噬细胞）的行为。在啮齿动物模型中，模拟人类患者神经损伤最常用的方法是坐骨神经横断和神经挤压，这两种方法都有成熟的脱髓鞘、免疫细胞流入、轴突再生和髓鞘再生时间进程。我们描述了坐骨神经横断和挤压损伤的手术技术，以及这两种模型中外周神经修复过程中事件分析的方法。
