Motohashi Tsutomu, Kunisada Takahiro
Department of Tissue and Organ Development, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine, Gifu, Japan.
Methods Mol Biol. 2019;1879:257-266. doi: 10.1007/7651_2018_144.
Melanoblasts (MBs) are melanocyte precursors that are derived from neural crest cells (NCCs). We recently demonstrated the multipotency of MBs; they differentiate not only into pigmented melanocytes but also other NCC derivatives. We herein describe methods for the isolation of MBs from mouse skin by flow cytometry. Methods to culture isolated MBs that retain their multipotency and isolation methods for MBs using gene-modified mouse are also described.
黑素母细胞（MBs）是源自神经嵴细胞（NCCs）的黑素细胞前体。我们最近证明了MBs的多能性；它们不仅能分化为有色素的黑素细胞，还能分化为其他NCC衍生物。本文描述了通过流式细胞术从小鼠皮肤中分离MBs的方法。还介绍了培养保留其多能性的分离MBs的方法以及使用基因修饰小鼠分离MBs的方法。
