Kosmas Konstantinos, Mitropoulou Georgia, Provatas Ioannis, Stamoulas Marios, Marouga Anna
Department of Cytopathology, General Chest Diseases Hospital of Athens "SOTIRIA", Athens, Greece.
Pathology Department, "Agia Sofia" Children's Hospital, Athens, Greece.
Cytopathology. 2018 Dec;29(6):558-564. doi: 10.1111/cyt.12612. Epub 2018 Aug 10.
The aims of this study were to determine the expression of phosphatase and tensin homologue (PTEN) in endometrial adenocarcinomas (as a potential prognostic indicator before treatment) in imprint smears and to correlate the results with clinicopathological parameters of primary untreated endometrial cancer patients.
A total of 126 patients with endometrial carcinoma were evaluated with samples freshly resected after a total abdominal hysterectomy during a 29-month period. The expression of PTEN was assessed by immunocytochemistry.
In total, 102 cases were type I and 24 type II endometrial adenocarcinomas. High expression of PTEN was more frequent in type I (42/102) compared to type II (6/24) adenocarcinomas, to less advanced and aggressive clinical stage (stage I: 41/79, stage II: 5/13, stage III: 2/19, stage IV: 1/15) as well as in low grade (grade 1: 26/42, grade 2: 20/57) compared to high-grade (grade 3: 8/27) carcinomas. The nonaffected lymph nodes showed high expression of PTEN (in 43.3%) than the affected lymph nodes (in 5.9%). Also, in 45 out of 74 cases with myometrial invasion <50%, there was positive expression of PTEN in contrast to 12 out of 52 cases with depth of myometrial invasion >50%.
Immunocytochemical findings from PTEN stain, in addition to cytomorphological features, appeared to be a useful marker in the diagnosis and in the postoperative prognosis of endometrial carcinoma in endometrial cytology with imprint smears and that high PTEN expression is related to morphological features of less aggressiveness tumours.
本研究旨在确定磷酸酶和张力蛋白同源物（PTEN）在子宫内膜腺癌印片涂片中的表达情况（作为治疗前的潜在预后指标），并将结果与未经治疗的原发性子宫内膜癌患者的临床病理参数相关联。
在29个月期间，对126例子宫内膜癌患者进行了评估，这些患者在全腹子宫切除术后切除了新鲜样本。通过免疫细胞化学评估PTEN的表达。
总共102例为I型子宫内膜腺癌，24例为II型。与II型腺癌（6/24）相比，PTEN高表达在I型腺癌（42/102）中更为常见，在临床分期较晚和侵袭性较强的患者中较少见（I期：41/79，II期：5/13，III期：2/19，IV期：1/15），并且在低级别（1级：26/42，2级：20/57）腺癌中比高级别（3级：8/27）腺癌更常见。未受影响的淋巴结中PTEN高表达（43.3%）高于受影响的淋巴结（5.9%）。此外，在74例肌层浸润<50%的病例中，有45例PTEN呈阳性表达，而在52例肌层浸润深度>50%的病例中，有12例呈阳性表达。
除细胞形态学特征外，PTEN染色的免疫细胞化学结果似乎是子宫内膜印片细胞学诊断和子宫内膜癌术后预后的有用标志物，并且PTEN高表达与侵袭性较小的肿瘤的形态学特征相关。
