Department of Biomolecular Science, Division of Toxicological, Hygiene and Environmental Science, University of Urbino Carlo Bo, Urbino, Italy.
Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Italy.
Food Res Int. 2018 Sep;111:472-479. doi: 10.1016/j.foodres.2018.05.067. Epub 2018 May 29.
The persistence of pathogenic bacteria in industrial settings is linked to biofilm embedded bacteria resistance to antimicrobial and disinfectant methods effective against planktonic cells. We proposed an experimental approach to evaluate sanitizers effectiveness against both planktonic microorganisms and related biofilms as possible integration of the official EN 1276 procedure. Firstly, the efficacy of three chemicals sanitizers was tested on planktonic cells of Escherichia coli O157:H7 ATCC 35150, Staphylococcus aureus ATCC 43387, Pseudomonas aeruginosa ATCC 9027, Enterococcus faecalis ATCC 29212 and Candida albicans ATCC 14053 using the suspension test indicated by EN 1276 in both dirty and clear simulated conditions (0.3% or 0.03% of bovine serum albumen). The sanitizers were tested against the related biofilms developed on stainless steel for 48 h at room temperature. The sanitizers (SANI 626, SUPERIG, IGIEN 155) reached 5-logarithmic reduction at the manufacture's recommended concentrations after 30 s and 5 min against planktonic microorganisms but, sometimes, the organic load interfered with their activity. The same concentrations tested with the proposed protocol weren't effective against biofilms and a log reduction >3 was reached using higher concentrations of the sanitizers and 15 min of contact, with the exception of IGIEN 155. The efficacy of a disinfectant/sanitizer is assessed against planktonic microorganisms and bacteria adhered to surface, while those embedded in biofilms are not taken into consideration. The proposed protocol could be used to evaluate the effectiveness of a sanitizer also against microorganisms organized in biofilms, in order to give to the users more detailed information on its activity.
工业环境中病原菌的持续存在与生物膜内细菌对抗微生物和消毒剂方法的抗药性有关，这些方法对浮游细胞有效。我们提出了一种实验方法来评估消毒剂对浮游微生物和相关生物膜的有效性，作为官方 EN 1276 程序的可能整合。首先，使用 EN 1276 指示的悬浮试验测试了三种化学消毒剂在大肠杆菌 O157:H7 ATCC 35150、金黄色葡萄球菌 ATCC 43387、铜绿假单胞菌 ATCC 9027、粪肠球菌 ATCC 29212 和白色念珠菌 ATCC 14053 的浮游细胞中的效力，模拟条件下（0.3%或 0.03%牛血清白蛋白）脏污和干净。消毒剂在室温下在不锈钢上培养 48 小时后用于测试相关生物膜。消毒剂（SANI 626、SUPERIG、IGIEN 155）在制造商推荐的浓度下作用 30 秒和 5 分钟后，对浮游微生物的减少达到 5 对数，但有机负荷有时会干扰其活性。用提议的方案测试相同浓度对生物膜无效，仅用消毒剂的更高浓度和 15 分钟的接触时间才能达到 >3 对数的减少，IGIEN 155 除外。消毒剂/杀菌剂的功效是根据浮游微生物和附着在表面上的细菌进行评估的，而那些嵌入生物膜中的细菌则不被考虑。所提出的方案可用于评估消毒剂对生物膜中微生物的有效性，以便为用户提供其活性的更详细信息。
