Gorbalenya A E, Blinov V M, Donchenko A P
FEBS Lett. 1986 Jan 6;194(2):253-7. doi: 10.1016/0014-5793(86)80095-3.
Here we demonstrate significant similarities between the amino acid sequences of trypsin (a serine protease) and the N-terminal piece of a specific fragment of the poliovirus polyprotein encompassing the sequence of the viral proteinase 3C, and also between cathepsin H (a cysteine protease) and the C-terminal piece of the same fragment. A coherent alignment of the sequences of the 3 proteases was obtained, in which the principal catalytically active residues occupy identical positions. A hypothesis is proposed that the viral enzyme may provide an evolutionary link between serine and cysteine protease families.
在此，我们证明了胰蛋白酶（一种丝氨酸蛋白酶）的氨基酸序列与脊髓灰质炎病毒多聚蛋白特定片段的N端部分（包含病毒蛋白酶3C的序列）之间存在显著相似性，并且组织蛋白酶H（一种半胱氨酸蛋白酶）与同一片段的C端部分之间也存在显著相似性。获得了这3种蛋白酶序列的连贯比对，其中主要的催化活性残基占据相同位置。提出了一种假说，即病毒酶可能在丝氨酸和半胱氨酸蛋白酶家族之间提供了一种进化联系。
