Chen Wenbo, Wang Kaimeng, Liu Shiyu
Department of Biology, Institute of Resources and Environment, Henan Polytechnic University, Jiaozuo 454000, Henan, China.
Department of Biology, Institute of Resources and Environment, Henan Polytechnic University, Jiaozuo 454000, Henan, China.
Comp Biochem Physiol A Mol Integr Physiol. 2018 Oct;224:68-75. doi: 10.1016/j.cbpa.2018.06.024. Epub 2018 Jul 2.
Fatty acid binding proteins (FABPs) are members of the conserved, multigene family of intracellular lipid binding proteins. In this study, the full-length cDNA of goldfish (Carassius auratus) FABP-3 (gfFABP-3) was successfully cloned. gfFABP-3 had an open reading frame of 402 bp and encoded a 133 amino acid polypeptide. The predicted gfFABP-3 protein included a lipocalin domain and displayed typical conserved FABP tertiary structures. Reverse transcription-PCR (RT-PCR) revealed that the gfFABP-3 gene was expressed in all tested tissues, with higher levels of expression in the testis, liver, heart, fat and kidney. After 24 h of cadmium exposure, gfFABP-3 was significantly upregulated in the gill, liver and spleen, but downregulated in the intestine, as compared to unexposed controls. gfFABP-3 expression was significantly downregulated in the spleen in goldfish challenged with LPS and Poly I:C. Our study provides a molecular characterization of goldfish FABP-3 and indicated that gfFABP-3 was potentially associated with the toxic effects of cadmium on lipid metabolism, and with the immune response to pathogenic infection.
脂肪酸结合蛋白（FABPs）是细胞内脂质结合蛋白保守多基因家族的成员。在本研究中，成功克隆了金鱼（Carassius auratus）FABP - 3（gfFABP - 3）的全长cDNA。gfFABP - 3具有402 bp的开放阅读框，编码一个133个氨基酸的多肽。预测的gfFABP - 3蛋白包含一个脂质运载蛋白结构域，并呈现典型的保守FABP三级结构。逆转录 - 聚合酶链反应（RT - PCR）显示，gfFABP - 3基因在所有测试组织中均有表达，在睾丸、肝脏、心脏、脂肪和肾脏中表达水平较高。与未暴露的对照组相比，镉暴露24小时后，gfFABP - 3在鳃、肝脏和脾脏中显著上调，但在肠道中下调。在用脂多糖（LPS）和聚肌苷酸 - 聚胞苷酸（Poly I:C）攻击的金鱼中，gfFABP - 3在脾脏中的表达显著下调。我们的研究提供了金鱼FABP - 3的分子特征，并表明gfFABP - 3可能与镉对脂质代谢的毒性作用以及对病原体感染的免疫反应有关。
