Chiasson R B, Carr B L
Gen Comp Endocrinol. 1985 Dec;60(3):427-33. doi: 10.1016/0016-6480(85)90077-2.
The pituitary of goitrogen-treated White Leghorn cockerels is smaller in size than control birds and the pituitaries of castrated cockerels is nearly twice the size of control birds. The pituitary cells generated by these treatments may not be functional thyrotrophs or gonadotrophs and may not be able to respond to their usual stimuli. Low ambient temperature is a well-known stimulus to the thyroid gland acting through pituitary TSH. Cyclic-AMP-dependent protein kinase activity levels are used here as an index of cellular activity in the pituitary and thyroid glands. Castrated cockerels with or without methimazole treatment do not have an increased pituitary cAMP-dependent protein kinase activity in cold. Methimazole-treated birds have an exaggerated pituitary protein kinase response to cold stress when compared with controls. Pituitary cAMP-dependent protein kinase activity is paralleled by a similar activity increase in the thyroid gland of methimazole-treated cockerels and no increase in the thyroid of castrated birds. Castrated birds at all temperatures have an elevated thyroid cAMP-dependent protein kinase activity ratio which is interpreted as the result of removal of testosterone inhibition.
用致甲状腺肿物质处理过的白来航公鸡的垂体比对照鸡的垂体小，而阉割公鸡的垂体大小几乎是对照鸡的两倍。这些处理产生的垂体细胞可能不是功能性促甲状腺激素细胞或促性腺激素细胞，可能无法对其通常的刺激作出反应。低环境温度是通过垂体促甲状腺激素作用于甲状腺的一种众所周知的刺激因素。在这里，环磷酸腺苷依赖性蛋白激酶活性水平被用作垂体和甲状腺细胞活性的指标。无论有无甲巯咪唑处理，阉割公鸡在寒冷环境中垂体环磷酸腺苷依赖性蛋白激酶活性都不会增加。与对照相比，用甲巯咪唑处理的鸡对冷应激的垂体蛋白激酶反应更为夸张。甲巯咪唑处理的公鸡甲状腺中类似的活性增加与垂体环磷酸腺苷依赖性蛋白激酶活性平行，而阉割鸡的甲状腺中没有增加。所有温度下阉割鸡的甲状腺环磷酸腺苷依赖性蛋白激酶活性比率都升高，这被解释为去除睾酮抑制的结果。
