Nedoszytko Bogusław, Wierzbicki Piotr, Karenko Leena, Maciejewska-Radomska Agata, Stachewicz Przemysław, Zabłotna Monika, Gleń Jolanta, Väkevä Liisa, Nowicki Roman J, Sokołowska-Wojdyło Małgorzata
Department of Dermatology, Venereology and Allergology, Medical University of Gdansk, Gdansk, Poland.
Department of Histology, Medical University of Gdansk, Gdansk, Poland.
Postepy Dermatol Alergol. 2018 Jun;35(3):274-279. doi: 10.5114/ada.2018.76224. Epub 2018 Jun 18.
Microbial infection and associated super antigens have been implicated in the pathogenesis of cutaneous T-cell lymphoma (CTCL), and many patients die from complicating bacterial infections. It has been postulated that () infection may be involved in the pathogenesis of (MF) but published data are limited and controversial.
To analyze the frequency of () DNA presence in blood samples of lymphoma cases.
Using Q-PCR method we analyzed the presence of DNA in the blood samples obtained from 57 patients with CTCL (55 - mycosis fungoides (MF)/Sézary syndrome (SS), one primary cutaneous anaplastic large cell lymphoma (CD30+) and one NKT cell lymphoma) and 3 patients with cutaneous B-cell lymphomas, and 120 individuals from control groups (40 patients with psoriasis, 40 patients with atopic dermatitis and 40 healthy controls).
DNA was identified in 13 of 55 cases in the MF/SS group (23.6%), in 1 patient with CD30+ large cell lymphoma and in 1 of 3 patients with B-cell lymphoma. The presence of was confirmed in 1 of 40 psoriatic patients (2.5%), in 5 of 40 patients with atopic dermatitis (12.5%) and in none of 40 healthy individuals. Presence of DNA in MF patients was strongly associated with disease progression; = 0.756; = 0.0123 for groups IA → IVB, and was noted more frequently in advanced (III + IV) stages than in early (I-II) stages ( = 0.0139). There are no differences in the mean age of MF/SS patients with and without infection.
The presence of DNA in the blood cells is a frequent event in late stages of MF/SS and may be explained by Th2 shift and suppression of the immune system during the course of the disease.
微生物感染及相关超抗原与皮肤T细胞淋巴瘤（CTCL）的发病机制有关，许多患者死于并发的细菌感染。据推测，（）感染可能参与蕈样肉芽肿（MF）的发病机制，但已发表的数据有限且存在争议。
分析淋巴瘤病例血样中（）DNA存在的频率。
我们采用Q-PCR方法分析了57例CTCL患者（55例蕈样肉芽肿（MF）/Sezary综合征（SS）、1例原发性皮肤间变性大细胞淋巴瘤（CD30+）和1例NKT细胞淋巴瘤）、3例皮肤B细胞淋巴瘤患者以及120名对照组个体（40例银屑病患者、40例特应性皮炎患者和40名健康对照）血样中DNA的存在情况。
MF/SS组55例中有13例（23.6%）检测到DNA，1例CD30+大细胞淋巴瘤患者和3例B细胞淋巴瘤患者中有1例检测到。40例银屑病患者中有1例（2.5%）检测到（），40例特应性皮炎患者中有5例（12.5%）检测到，40名健康个体均未检测到。MF患者中（）DNA的存在与疾病进展密切相关；IA→IVB组的（相关系数）=0.756；（P值）=0.0123，且在晚期（III+IV期）比早期（I-II期）更频繁地检测到（P值=0.0139）。有无感染的MF/SS患者平均年龄无差异。
血细胞中（）DNA的存在在MF/SS晚期较为常见，这可能是由于疾病过程中Th2偏移和免疫系统抑制所致。
