Chen Hongbing, Wang Siping
Department of Gastrointenstinal Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China.
Department of Emergency, Yantaishan Hospital, Yantai, Shandong 264001, P.R. China.
Oncol Lett. 2018 Aug;16(2):1483-1490. doi: 10.3892/ol.2018.8838. Epub 2018 May 30.
ADAM metallopeptidase domain 29 (ADAM29) belongs to the ADAM family, is a type I integral membrane protein and secrets a glycoprotein that mediates cell-cell and cell-matrix interaction. Aberrant expression of ADAM29 is involved in a number of physiological processes diversification. The purpose of the present study was to investigate the expression and biological effect of ADAM29 in human gastric cancer (GC) specimens and cell lines . The expression of ADAM29 was examined in 83 GC samples and 25 adjacent normal gastric tissues using quantitative reverse transcriptase-quantitative polymerase chain reaction and immunohistochemistry. The association between ADAM29 expression and cellular function of GC cells was assessed . The ADAM29 mRNA levels were significantly elevated in GC tissues compared with paracancerous tissues. Increased levels of ADAM29 were associated with high-grade staging and high Tumor-Node-Metastasis stages. Kaplan-Meier survival curves demonstrated that patients with GC and low ADAM29 transcript levels exhibited longer overall survival (OS) (P<0.01) and progression-free survival (PFS) time (P<0.01) compared with patients with high ADAM29 expression levels. ADAM29 significantly promoted the proliferation, migration and invasion of GC cells when overexpressed in MGC803 cells and knocked down in AGS cells. ADAM29 was increased in GC and the elevated expression of ADAM29 was associated with a poor survival rate of patients. ADAM29 may become a prognostic factor and therapeutic candidate for human GC.
ADAM金属蛋白酶结构域29（ADAM29）属于ADAM家族，是一种I型整合膜蛋白，可分泌介导细胞间和细胞与基质相互作用的糖蛋白。ADAM29的异常表达参与了许多生理过程的多样化。本研究的目的是探讨ADAM29在人胃癌（GC）标本和细胞系中的表达及生物学效应。采用定量逆转录-定量聚合酶链反应和免疫组织化学方法检测83例GC样本和25例癌旁正常胃组织中ADAM29的表达。评估ADAM29表达与GC细胞功能之间的关联。与癌旁组织相比，GC组织中ADAM29 mRNA水平显著升高。ADAM29水平升高与高分级分期和高肿瘤-淋巴结-转移分期相关。Kaplan-Meier生存曲线显示，与ADAM29高表达水平的患者相比，GC且ADAM29转录水平低的患者总生存期（OS）更长（P<0.01），无进展生存期（PFS）时间更长（P<0.01）。当在MGC803细胞中过表达并在AGS细胞中敲低时，ADAM29显著促进GC细胞的增殖、迁移和侵袭。GC中ADAM29升高，且ADAM29表达升高与患者生存率低相关。ADAM29可能成为人类GC的预后因素和治疗靶点。
