Guan Bing, Mu Lijun, Zhang Linlin, Wang Ke, Tian Juanhua, Xu Shan, Wang Xinyang, He Dalin, Du Yuefeng
Department of Urology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.
Oncol Lett. 2018 Aug;16(2):1821-1826. doi: 10.3892/ol.2018.8877. Epub 2018 Jun 1.
MicroRNA (miRNA) is a class of non-coding single-stranded RNA, able to regulate tumor-associated genes via binding the 3'-UTR of the target gene mRNA. Previous publications have demonstrated that miRNA-218 (miR-218) acts as a tumor-suppressive miRNA in various types of human cancer, including prostate cancer (PCa). However, the role of miR-218 in regulating PCa cell stemness and epithelial-mesenchymal transition remains unknown and requires further research. In the present study, it is demonstrated that miR-218 was downregulated in 2 PCa cell lines and could suppress cell migration, EMT and the exhibition of cancer stem cell-like properties. The expression of GLI family zinc finger 1 (Gli1) was inhibited by miR-218 overexpression, suggesting miR-218-suppression of Gli1 as a potential mechanism for the tumor-suppressive effect of miR-218. Overall, the results indicate that miR-218 served a critical role in the inhibition of PCa development. This may provide new insight for elucidating the mechanisms of PCa oncogenesis and suggests that miR-218 may be a novel therapeutic target for PCa.
微小RNA（miRNA）是一类非编码单链RNA，能够通过结合靶基因mRNA的3'-非翻译区来调控肿瘤相关基因。先前的研究表明，miRNA-218（miR-218）在包括前列腺癌（PCa）在内的多种人类癌症中作为一种肿瘤抑制性miRNA发挥作用。然而，miR-218在调节PCa细胞干性和上皮-间质转化中的作用尚不清楚，需要进一步研究。在本研究中，证明miR-218在2种PCa细胞系中表达下调，并且能够抑制细胞迁移、上皮-间质转化以及癌症干细胞样特性的表现。miR-218过表达抑制了GLI家族锌指蛋白1（Gli1）的表达，提示miR-218对Gli1的抑制作用可能是其发挥肿瘤抑制作用的潜在机制。总体而言，结果表明miR-218在抑制PCa发展中起关键作用。这可能为阐明PCa发生机制提供新的见解，并提示miR-218可能是PCa的一个新的治疗靶点。
