Suryandari Dwi Anita, Midoen Yurnadi Hanafi, Yunaini Luluk, Setyaningsih Sari, Freisleben Hans-Joachim
Department of Medical Biology, Faculty of Medicine, Universitas Indonesia, Jakarta Pusat, Indonesia.
Master Program Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta Pusat, Indonesia.
J Reprod Infertil. 2018 Apr-Jun;19(2):100-108.
Spermatogenesis is a tightly regulated developmental process of male germ cells. The stages in spermatogenesis are mitosis, meiosis and spermiogenesis. One of the genes playing a role in meiosis is Cell Division Cycle 25A (CDC25A). Decreased expression of CDC25A is associated with failure of spermatogenesis and sperm retrieval. Infertility examination for azoospermia has been limited on histological examination. Hence, molecular research to find marker genes for infertility will improve the examination of testis biopsies.
This research is a cross sectional study of 50 testicular biopsies with Johnsen scoring categories from scoring 2 to 8. Analysis of mRNA expression used qPCR and protein expression using immunohistochemistry. Statistical analysis with Spearman correlation was considered significant at p<0.05.
The result showed that transcript level and protein expression of CDC25A decreased in score 5 of Johnsen scoring categories. Moderate Spearman rho correlation (r=0.546) between mRNA relative expression and protein expression of CDC25A was significant at p<0.01.
Decreased expression of CDC25A is associated with meiotic arrest as the etiology of spermatogenic failure in many azoospermic men.
精子发生是男性生殖细胞一个受到严格调控的发育过程。精子发生的阶段包括有丝分裂、减数分裂和精子形成。在减数分裂中发挥作用的基因之一是细胞分裂周期25A（CDC25A）。CDC25A表达降低与精子发生失败和取精失败有关。无精子症的不育检查在组织学检查方面一直存在局限性。因此，寻找不育标记基因的分子研究将改善睾丸活检检查。
本研究是一项横断面研究，对50例睾丸活检样本进行约翰森评分，评分范围为2至8分。使用定量聚合酶链反应（qPCR）分析mRNA表达，并使用免疫组织化学分析蛋白质表达。采用Spearman相关性统计分析，p<0.05被认为具有统计学意义。
结果显示，在约翰森评分5分的类别中，CDC25A的转录水平和蛋白质表达降低。CDC25A的mRNA相对表达与蛋白质表达之间存在中度Spearman等级相关性（r=0.546），p<0.01具有统计学意义。
在许多无精子症男性中,CDC25A表达降低与减数分裂阻滞有关，是精子发生失败的病因。
