Haraguchi Yuji, Matsuura Katsuhisa, Kagawa Yuki, Hasegawa Akiyuki, Kubo Hirotsugu, Shimizu Tatsuya
Institute of Advanced Biomedical Engineering and Science, TWIns, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.
Ogino Memorial Laboratory, Nihon Kohden Corporation, TWIns, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.
Biotechnol Prog. 2018 Nov;34(6):1447-1453. doi: 10.1002/btpr.2691. Epub 2018 Oct 2.
This study reports a rapid fabrication system of a morphologically and functionally communicative three-dimensional (3D) cell-dense tissue without scaffolds by centrifugation. The tight adhesion between C2C12 myoblasts and culture surface was accelerated without significant cell damage by centrifugation (80 x g, 37 °C, 30 min). A thicker tissue created on a temperature-responsive culture surface was harvested by decreasing temperature. The 3D myoblast tissues having approximately 200 μm-thickness were created at 1.5 h [centrifugation (80 x g, 37 °C) for 30 min and tissue harvest for 1 h]. However, in the case of without centrifugation, the myoblast tissues had fragile parts even at 7.5 h after the incubation. Additionally, electrically/functionally communicative and thicker human induced pluripotent stem (iPS) cell-derived cardiac tissues were created rapidly by the centrifugation and cultivation at 37 °C. We report a centrifugation system that significantly shortens the creation time of 3D tissues. We envision that this procedure will contribute to the field of tissue engineering and regenerative medicine. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:1447-1453, 2018.
本研究报道了一种通过离心快速制造无支架的形态和功能可通信三维（3D）细胞密集组织的系统。通过离心（80×g，37°C，30分钟），C2C12成肌细胞与培养表面之间的紧密粘附得以加速，且细胞无明显损伤。通过降低温度，收获在温度响应性培养表面上形成的更厚组织。在1.5小时内（离心（80×g，37°C）30分钟，组织收获1小时）创建了厚度约为200μm的3D成肌细胞组织。然而，在不进行离心的情况下，即使在孵育7.5小时后，成肌细胞组织仍有脆弱部分。此外，通过离心和在37°C下培养，快速创建了电/功能可通信且更厚的人诱导多能干细胞（iPS）衍生的心脏组织。我们报道了一种显著缩短3D组织创建时间的离心系统。我们设想该方法将对组织工程和再生医学领域做出贡献。©2018美国化学工程师学会生物技术进展，34:1447 - 1453，2018。
