使用酶标板检测全血中伪狂犬病病毒抗体。
Detection of antibodies to Aujeszky's disease virus in whole blood by Elisadisc.
作者信息
Banks M
出版信息
J Virol Methods. 1985 Oct;12(1-2):41-5. doi: 10.1016/0166-0934(85)90006-0.
PMID:3001121
Abstract
A rapid method for the detection of antibodies in whole blood by ELISA was developed. A punched filter paper disc configuration, the Elisadisc, was loaded with blood, dried, and applied directly to the Aujeszky's disease ELISA, with minimal pretreatment. Rapidity, throughput and sensitivity were equal to that of the serum ELISA. No loss of activity could be detected in the discs after storage at +4 degrees C for almost 1 yr.
摘要
开发了一种通过酶联免疫吸附测定（ELISA）快速检测全血中抗体的方法。一种打孔滤纸圆盘结构，即酶联免疫吸附测定圆盘（Elisadisc），加载血液后干燥，无需太多预处理即可直接用于伪狂犬病ELISA检测。其速度、通量和灵敏度与血清ELISA相当。在4摄氏度下储存近1年后，圆盘上未检测到活性损失。
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