Yan Ting Ting, Zhang Lei, Li Yu Dong, Liang Xin le
College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China.
Yi Chuan. 2018 Jul 20;40(7):601-606. doi: 10.16288/j.yczz.18-018.
With the rapid development of the internet technology, the blended learning mode plays a more and more important role in education reforms by integrating traditional classroom teaching and online learning. WeChat is the most popular Chinese social software, and its public platform is also suitable for mobile learning. Here, we report an application of the blended learning method based on WeChat public platforms in microbiology breeding experiment courses, using the site-directed mutagenesis of the green fluorescent protein (GFP) as an example. The learning process was divided into five modules: teaching design, learning resource preparation, pre-class learning, classroom learning, post-class review and evaluation. By introducing one mutation (Y66H) in mutagenic primers, the mutated GFP gene was amplified by PCR using pGFPuv as templates, followed by removal of the original plasmid template by Dpn1 digestion. Students can monitor the color changes from green to blue in the fluorescence emission of the mutated proteins. As a useful addition to classroom teaching, WeChat is suitable for students to use fragmented time to learn and improve teaching interaction. Learning assessment results revealed the blended learning environment improves students' study interests and self-learning abilities, thus achieving a fruitful teaching result.
随着互联网技术的飞速发展，混合学习模式通过整合传统课堂教学和在线学习在教育改革中发挥着越来越重要的作用。微信是中国最受欢迎的社交软件，其公众号平台也适用于移动学习。在此，我们报告基于微信公众号平台的混合学习方法在微生物育种实验课程中的应用，以绿色荧光蛋白（GFP）的定点突变为例。学习过程分为五个模块：教学设计、学习资源准备、课前学习、课堂学习、课后复习与评价。通过在诱变引物中引入一个突变（Y66H），以pGFPuv为模板通过PCR扩增突变的GFP基因，随后用Dpn1消化去除原始质粒模板。学生可以监测突变蛋白荧光发射中从绿色到蓝色的颜色变化。作为课堂教学的有益补充，微信适合学生利用碎片化时间学习并提高教学互动性。学习评估结果表明，混合学习环境提高了学生的学习兴趣和自主学习能力，从而取得了丰硕的教学成果。
