Institute of Physiology, University Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland.
Pflugers Arch. 2019 Jan;471(1):67-82. doi: 10.1007/s00424-018-2176-2. Epub 2018 Jul 18.
The sorting and stabilization of proteins at specific subcellular domains depend upon the formation of networks build up by specific protein-protein interactions. In addition, protein networks also ensure the specificity of many regulatory processes by bringing together regulatory molecules with their targets. Whereas the success on the identification of protein-protein interactions is (up to a point) technology-driven, the assignment of functional roles to specific partners remains a major challenge. This review summarizes the work that led to the identification of partners of the Na/phosphate cotransporter NaPi-IIa as well as the effects of the interactions in the expression and/or regulation of the cotransporter.
蛋白质在特定亚细胞区域的分拣和稳定依赖于由特定蛋白-蛋白相互作用形成的网络的构建。此外，蛋白质网络还通过将调节分子与其靶标聚集在一起，确保许多调节过程的特异性。虽然蛋白质-蛋白质相互作用的鉴定在一定程度上是技术驱动的，但将特定伙伴的功能角色分配仍然是一个主要挑战。这篇综述总结了导致鉴定 Na/磷酸盐共转运蛋白 NaPi-IIa 的伙伴以及这些相互作用对共转运蛋白表达和/或调节的影响的工作。
