Shinohara O, Knecht M, Catt K J
Biochem Biophys Res Commun. 1985 Dec 17;133(2):468-74. doi: 10.1016/0006-291x(85)90930-1.
Hormonal induction of granulosa cell maturation is inhibited by phorbol esters and permeant synthetic diacylglycerols, but these activators of protein kinase C differ in their effects on cAMP production and actions. Both agents prevented the induction of luteinizing hormone receptors and progesterone biosynthesis by follicle-stimulating hormone, choleragen, and forskolin, but only diacylglycerol abolished the cAMP responses to these stimuli. Granulosa cell aggregation and aromatase activity were inhibited by phorbol ester but not completely by diacylglycerol. In intact granulosa cells, cytosolic C kinase activity was rapidly decreased by phorbol ester but unaffected by diacylglycerol. Although diacylglycerol has a marked inhibitory action on cAMP production, the more prominent suppression of granulosa cell differentiation by phorbol ester may be related to its rapid and prolonged action on kinase C.
佛波酯和可渗透的合成二酰基甘油可抑制激素诱导的颗粒细胞成熟，但这些蛋白激酶C激活剂对环磷酸腺苷（cAMP）产生和作用的影响有所不同。这两种试剂均可阻止促卵泡激素、霍乱毒素和福斯高林诱导促黄体生成素受体和孕酮生物合成，但只有二酰基甘油消除了对这些刺激的cAMP反应。佛波酯可抑制颗粒细胞聚集和芳香化酶活性，但二酰基甘油不能完全抑制。在完整的颗粒细胞中，佛波酯可迅速降低胞质C激酶活性，但二酰基甘油对其无影响。尽管二酰基甘油对cAMP产生有显著抑制作用，但佛波酯对颗粒细胞分化更显著的抑制作用可能与其对激酶C的快速和持久作用有关。
