Beijing Neurosurgical Institute, Capital Medical University, Beijing 100050, China.
Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.
Biomed Environ Sci. 2018 Jun;31(6):413-424. doi: 10.3967/bes2018.055.
To investigate the optimal timing for the repair of persistent incomplete facial paralysis by hypoglossal-facial 'side'-to-side neurorrhaphy in rats.
A total of 30 adult rats with crushed and bulldog-clamped facial nerve injury were randomly divided into 5 groups (n = 6 each) that were subjected to injury without nerve repair or with immediate repair, 2-week-delayed repair, 4-week-delayed repair, or 8-week-delayed repair. Three months later, the effects of repair in each rat were evaluated by facial symmetry assessment, electrophysiological examination, retrograde labeling, and axon regeneration measurement.
At 3 months after injury, the alpha angle significantly increased in the group of rats with 4-week-delayed repair compared with the other four groups. Upon stimulation of the facial nerve or Pre degenerated nerve, the muscle action potentials MAPs were recorded in the whisker pad muscle, and the MAP amplitude and area under the curve in the 4-week-delayed repair group were significantly augmented at 3 months post-injury. Similarly, the number of retrograde-labeled motor neurons in the facial and hypoglossal nuclei was quantified to be significantly greater in the 4-week-delayed repair group than in the other groups, and a large number of regenerated axons was also observed.
The results of this study demonstrated that hemiHN-FN neurorrhaphy performed 4 weeks after facial nerve injury was most effective in terms of the functional recovery of axonal regeneration and activation of facial muscles.
探讨舌下神经-面神经侧侧吻合术修复大鼠持续性不完全性面瘫的最佳时机。
将 30 只成年大鼠的面神经挤压伤和夹伤后，随机分为 5 组（每组 6 只），分别为未修复神经、即时修复、2 周延迟修复、4 周延迟修复和 8 周延迟修复。3 个月后，通过面部对称性评估、电生理检查、逆行标记和轴突再生测量来评价每组大鼠的修复效果。
损伤后 3 个月，4 周延迟修复组大鼠的 α 角明显增大。刺激面神经或预变性神经时，可在须垫肌记录到肌动作电位（MAPs），4 周延迟修复组在损伤后 3 个月时 MAP 幅度和曲线下面积明显增加。同样，面神经核和舌下神经核内逆行标记的运动神经元数量也明显多于其他组，且观察到大量再生轴突。
本研究结果表明，面神经损伤后 4 周进行舌下神经-面神经侧侧吻合术在促进轴突再生和面部肌肉激活的功能恢复方面最为有效。
