使用3D培养系统从牙髓干细胞再生胰岛素分泌胰岛。
Regeneration of insulin-producing islets from dental pulp stem cells using a 3D culture system.
作者信息
Yagi Mendoza Hiromi, Yokoyama Tomomi, Tanaka Tomoko, Ii Hisataka, Yaegaki Ken
机构信息
Department of Oral Health, School of Life Dentistry at Tokyo, Nippon Dental University, 1-9-20, Fujimi, Chiyoda ku, 102-8159 Tokyo, Japan.
出版信息
Regen Med. 2018 Sep;13(6):673-687. doi: 10.2217/rme-2018-0074. Epub 2018 Jul 20.
AIM
In this study, we aimed to establish the differentiation protocol of dental pulp stem cells (DPSCs) into pancreatic islets using a 3D structure.
MATERIALS & METHODS: DPSCs were differentiated in a 3D culture system using a stepwise protocol. Expression of β-cell markers, glucose-stimulated insulin secretion, and PI3K/AKT and WNT pathways were compared between monolayer-cultured pancreatic cells and islets.
RESULTS
Islet formation increased insulin and C-peptide production, and enhanced the expression of pancreatic markers. Glucose-dependent secretion of insulin was increased by islets. Pancreatic endocrine markers, transcriptional factors, and the PI3K/AKT and WNT pathways were also upregulated.
CONCLUSION
Pancreatic islets were generated from DPSCs in a 3D culture system. This system could provide novel strategies for controlling diabetes through regenerative medicine.
目的
在本研究中，我们旨在利用三维结构建立牙髓干细胞（DPSCs）向胰岛分化的方案。
材料与方法
使用逐步方案在三维培养系统中诱导DPSCs分化。比较单层培养的胰腺细胞和胰岛中β细胞标志物的表达、葡萄糖刺激的胰岛素分泌以及PI3K/AKT和WNT信号通路。
结果
胰岛形成增加了胰岛素和C肽的产生，并增强了胰腺标志物的表达。胰岛增加了胰岛素的葡萄糖依赖性分泌。胰腺内分泌标志物、转录因子以及PI3K/AKT和WNT信号通路也上调。
结论
在三维培养系统中，DPSCs可分化生成胰岛。该系统可为通过再生医学控制糖尿病提供新策略。
Zotero 插件