Green R D, Frelin C, Vigne P, Lazdunski M
FEBS Lett. 1986 Feb 3;196(1):163-6. doi: 10.1016/0014-5793(86)80234-4.
When chick cardiac cells are maintained in serum-free NCTC-135 medium, the pHi dependence of the Na+/H+ exchanger is much more acidic (pK = 7.0) than when a serum-free M199 medium is used (pK greater than 7.5). Phorbol esters activate the Na+/H+ exchanger and produce a cell alkalinization in cells maintained in NCTC-135 medium by shifting the pHi dependence of the system to more alkaline values. They have no action on cells maintained in M199 medium. The alkaline pHi dependence of the Na+/H+ exchanger and the loss of responsiveness of phorbol ester action in M199 medium correlate with increased rates of phosphatidylinositol turnover.
当鸡心脏细胞在无血清的NCTC - 135培养基中培养时，钠氢交换体对细胞内pH值（pHi）的依赖性比在无血清的M199培养基中培养时更偏酸性（pK = 7.0）（在M199培养基中pK大于7.5）。佛波酯激活钠氢交换体，并通过将该系统对pHi的依赖性向更碱性的值移动，使在NCTC - 135培养基中培养的细胞发生细胞碱化。它们对在M199培养基中培养的细胞没有作用。钠氢交换体对pHi的碱性依赖性以及在M199培养基中佛波酯作用反应性的丧失与磷脂酰肌醇周转率的增加相关。
